化疗药物对胰腺癌细胞株SW-1990蛋白质谱表达的影响

Effect of chemotherapy drug on protein expression of pancreas cancer cell lines SW-1990

目的探讨化疗药物作用下胰腺癌细胞株蛋白质谱的变化,寻找差异表达的蛋白质,为临床化疗提供理论依据。方法利用蛋白质组学技术,观察胰腺癌细胞株SW-1990经吉西他滨和5-氟尿嘧啶(5-FU)处理后,其蛋白质谱的变化,鉴定其差异表达的蛋白质。结果培养细胞受抑制约50％时,吉西他滨为1-100ng／ml。5-FU为250-2500 ng／ml。质谱鉴定了6个差异表达的蛋白质,对照组1个,加药组5个。结论对细胞株的作用,吉西他滨明显优于5-FU。吉西他滨参与了糖代谢和酯类代谢。β-肌动蛋白、MGC:19713、KIAA1058蛋白等某些高表达的差异蛋白质可能成为观察化疗效果的重要指标。

Objectives To verify if chemotherapy drug gemcitabine could induce a change of protein expression in pancreatic cancer cell line SW-1990, and to provide evidence for clinical therapy. Methods We use two-dimensional gel electrophoresis（2-DE）, mass spectrometry and bio-information methods to compare the change of protein expression before and after gemcitabine and 5-FU treatment of cell line SW-1990, and identify the discrepant protein expression. Results The concentration of gemcitabine, that can suppress 50 percent of the pancreatic cancer cells was 1-100 ng/ml, while same suppression for 5-FU was 250-2500 ng/ml. Six discrepant protein expressions, one in 5-FU group and 5 in gemcitabine group were found. Conclusions Gelaacitabine has better therapeutic effects on the growth of pancreatic cancer cell line SW-1990 than that of 5-FU. Gemcitabine was involved in sugar and fat metabolism, and it could induce some changes of protein expression, for example β-actin and MGC： 19713, which is of somewhat importance for clinical investigation of pancreatic cancer therapy.