前S_1蛋白和前S_2蛋白及HBV-DNA平行检测对HBV感染诊断的临床价值

Clinical signification of detecting Pre-S_1 protein and Pre-S_2 protein in diagnosis of hepatitis B virus infection

目的:探讨前S1蛋白和前S2蛋白联合诊断乙肝病毒感染复制的临床意义。方法:应用ELISA技术和PCR方法对216例HBsAg阳性标本进行前S1蛋白、前S2蛋白及HBV-DNA平行检测。结果:在“大三阳”组中,前S1蛋白、前S2蛋白及HBV-DNA检出阳性率分别为69．5％、42．9％和86．7％;在109例HBeAg阴性血清中,前S1蛋白39例(35．8％)、前S2蛋白5例(4．6％)及HBV-DNA14例(12．8％)检出阳性。前S1蛋白与HBV- DNA的阳性率比较差异无统计学意义(x2=0．613,P>0．250),与HBeAg的阳性率比较差异无统计学意义(x2= 0．493,P>0．250)。前S2蛋白与HBV-DNA阳性率比较差异有统计学意义(x2=40．36,P<0．005),与HBeAg 阳性率比较差异有统计学意义(x2=46．81,P<0．005)。结论:前S1蛋白和前S2蛋白是诊断乙肝病毒感染复制十分有价值的血清学指标。

Objective： To investigate the clinical significance of detection Pre-S1 protein and Pre-S2 protein in diagnosis of HBV infection. Methods： Using PCR to detect HBV-DNA and ELISA technique to detect Pre- S1 protein and Pre-S2 protein for 216 samples with HBsAg positive in the meantime. Results： Pre-S1 protein, Pre-S2 protein and HBV-DNA positive rates of sample with positive HBsAg, HBeAg, HBcAb markers were 69.5%, 42.9% and 86.7% respectively; Prs-S1 protein, Pre-S2 protein and HBV-DNA were positive in 39 （35.8%） cases, 5 （4.6%） cases and 14 （12.8%） cases in the 109 cases serum samp ative HBeAg respectively. There was no significant difference between Pre-S1 protein and HBV-DNA （X^2=0. 613, P 〉0. 250）. There was no significant difference between Pre-S1 protein and HBeAg by X^2 test （X^2=0. 493, P 〉0. 250）. There was significant difference between Pre-S2 protein and HBV-DNA （X^2=40.36, P〈0. 005） ,There was significant difference between Pre-S2 protein and HBeAg （X^2 =46.81, P〈0. 005）. Conclusion： Pre-S1 protein and pre-S2 protein are both great applying valuably serological marker in clinical diagnosis of HBV infection and replication.