期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

copine Ⅴ蛋白的亚细胞定位 被引量：6

Subcellular Localization of copine Ⅴ
下载PDF
导出
摘要 目的:确定copineⅤ蛋白的亚细胞定位,初步研究该蛋白的生物学功能。方法:将copineⅤ编码区基因分别构建真核表达载体pEGFP-copineⅤ(或pRED-copineⅤ),转染HEK293、HeLa细胞,在激光共聚焦荧光显微镜下与转染空载体pEGFP-N1(或pRED-N1)的细胞比较观察。结果:经限制性内切酶分析鉴定,构建的重组表达载体正确。通过激光共聚焦荧光显微镜观察,转染了重组载体pEGFP-copineⅤ的细胞荧光信号集中分布于胞膜和内膜系统;进一步研究表明copineⅤ定位于内质网而非线粒体,而空载体则在整个细胞中均匀分布。结论:copineⅤ蛋白定位于细胞膜和内质网上,而不定位于线粒体。 Objective： To investigate the subcellular localization of copine V gene. Methods： The expressive vector of pEGFP-copineV （or pRED-copineV ） was constructed by routine molecular biological, HEK293 and HeLa cells were transfected with pEGFP-N1 and pEGFP-copineV, respectively by using LipofectAMINE2000. The expression was dectected by fluorescence microscope and confocal fluorescence microscope. Results： The expression vectors were confirmed by re- strietion enzyme disgestion. The green and red fluorescence could be seen in cell membrane and endoplasmic reticulum rather than mitochondrial in transfected copine V gene cells and were distributed uniformly in whole cell in transfected pEGFP-N1 （or pRED-N1 ） cells respectively. Conclusion： The expression of copineV gene was distributed in cell membrane and endoplasmic reticulum rather than mitochondrial.
作者 王晓文 靳雁斌 吴燕 吴彦瑞 马鑫 刘淑红 范文红 范明
机构地区 军事医学科学院基础医学研究所
出处 《生物技术通讯》 CAS 2006年第2期149-151,共3页 Letters in Biotechnology
基金 国家高技术研究发展计划课题(2002BA711A01-03)
关键词 copine V蛋白 亚细胞定位 内质网 线粒体 细胞膜 copineV protein subcellular localizalion endoplasmic reticulum mitochondrial cytoplasma membrane
分类号 Q25 [生物学—细胞生物学]
  • 相关文献

参考文献9

  • 1Cowland JB,Carter D,Bjerregaard MD,et al.Tissue expression of copines and isolation of copines Ⅰ and Ⅲ from the cytosol of human neutrophils[J].J Leukoc Biol,2003,74(3):379.
  • 2Hua J,Grisafi P,Cheng SH,et al.Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes [J].Genes Dev,2001,15(17):2263.
  • 3Jambunathan N,Siani JM,McNellis TW.A humidity-sensitive Arabidopsis copine mutant exhibits precocious cell death and increased disease resistance[J].Plant Cell,2001,13(10):2225.
  • 4J ambunathan N,McNellis TW.Regulation of Arabidopsis copine 1gene expression in response to pathogens and abiotic stimuli [J].Plant Physiol,2003,132(3):1370.
  • 5Church DL,Lambie EJ.The promotion of gonadal cell divisions by the Caenorhabditis elegans TRPM cation channel GON-2 is antagonized by GEM-4 copine[J].Genetics,2003,165(2):563.
  • 6Tomsig JL,Sohma H,Creutz CE,et al.Calcium-dependent regulation of tumour necrosis factor-alpha receptor signalling by copine[J].Biochem J,2004,378(15):1089.
  • 7Caudell EG,Caudell JJ,Tang CH,et al.Characterization of human copine Ⅲ as a phosphoprotein with associated kinase activity [J].Biochemistry,2000,39(42):13034.
  • 8王晓文,靳雁斌,景孝堂,吴燕,马鑫,刘淑红,范文红,范明.外源表达的人copine Ⅴ诱导细胞死亡的研究[J].生物技术通讯,2006,17(2):146-148. 被引量：6
  • 9王晓文,范文红,范明.Copines蛋白家族的研究进展[J].国外医学（生理病理科学与临床分册）,2005,25(2):153-155. 被引量：7

二级参考文献27

  • 1Nakayama T, Yaoi T, Yasui M, et al. N-eopine:a novel two C2-domain-containing protein with neuronal activity-regulated expression[J]. FESS Lett,1998,428(1-2) :80-84.
  • 2Nakayama T, Yaoi T, Kuwajima G. Localization and subcellular distribution of N-copine in mouse brain [ J ]. J Neurochem, 1999,72( 1 ) :373-379.
  • 3Maitra R, Grigoryev DN, Bera TK,et al. Cloning, molecular characterization, andexpression analysis of Copine 8 [J]. Biochem Biophys Res Commun,2003,303 (3) :842-847.
  • 4Savino M, d'Apolito M, Centra M, et al. Characterization of copine Ⅶ, a new member of the copine family, and its exclusion as a candidate in sporadic breast cancers with loss of heterozygosity at 16q24.3 [J]. Genomics,1999,61(2):219-226.
  • 5Ramsey H, Zhang DE, Richkind K, et al. Fusion of AML1/Runxl to copine VIII, a novel member of the copine family, in an aggressive acute myelogenous leukemia with t( 12 ;21 ) translocation[J].Leukemia, 2003,17(8) :1665-1666.
  • 6Hua J, Gfisafi P, Cheng SH, et al. Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes[J]. Genes Dev,2001.15 (17):2263-2272.
  • 7Jambunathan N, Siani JM, McNellis TW. A humidity-sensitive Arabidopsis copine mutant exhibits precocious cell death and increased disease resistance [J]. Plant Cell, 2001,13 (10) :2225-2240.
  • 8Jambunathan N, McNellis TW. Regulation of Arabidopsis COPINE 1 gene expression in response to pathogens and abiotic stimuli [J].Plant Physiol, 2003,132 ( 3 ) : 1370-1381.
  • 9Church DL, Lambie EJ. The promotion of gonadal cell divisions by the Caenorhabditis elegans TRPM cation channel GON-2 is antagonized by GEM -4 copine [J]. Genetics, 2003,165 ( 2 ) :563 -574.
  • 10Nakayama T, Yaoi T, Kuwajima G, et al. Ca^2+ -dependent interaction of N-copine, a member of the two C2 domain protein family,with OS-9, the product of a gene frequently amplified in osteosarcoma[J]. FEBS Lett, 1999,453 (1-2) :77-80.

共引文献11

同被引文献52

  • 1唐元家,邹洪,谢青莲,金玫蕾.N-copine基因反义核酸对原代培养的小鼠皮质神经元的影响[J].中国病理生理杂志,2006,22(1):80-83. 被引量：3
  • 2王晓文,靳雁斌,景孝堂,吴燕,马鑫,刘淑红,范文红,范明.外源表达的人copine Ⅴ诱导细胞死亡的研究[J].生物技术通讯,2006,17(2):146-148. 被引量：6
  • 3Creutz CE, Tomsig JL, Snyder SL, et al. The copines, a novel class of C2 domain-containing, calcium-dependent, phospholipid-binding proteins conserved from Paramecium to humans[J]. J Biol Chem, 1998, 273(3):1393-1402.
  • 4Rizo J, Sudhof TC. C2-domains, structure and function of a universal Ca^2+-binding domain[J]. J Biol Chem, 1998, 273(26):15879-15882.
  • 5Wang CY, Mayo MW, Baldwin AS Jr. TNF-α and cancer therapy-induced apoptosis: potentiation by inhibition of NF-κB[J]. Science, 1996, 274(5288):784-787.
  • 6Tomsig JL, Sohma H, Creutz CE. Calcium-dependent regulation of tumour necrosis factor-alpha receptor signalling by copine[J]. Biochem J, 2004, 378(15): 1089-1094.
  • 7Henkel T, Machleidt T, Alkalay I, et al. Rapid proteolysis of Ⅰ kappa B-α is necessary for activation of transcription factor NF-kappa B[J]. Nature, 1993, 365(6442):182-185.
  • 8Creutz CE,Tomsig JL,Snyder SL,et al.The copinos,a novel class of C2 domain-containing,calcium-dependent,phospholipid-binding proteins conserved from Paramecium to humans[J].J Biol Chem,1998,273(3):1393-1402.
  • 9Sen R,Baltimore D.Multiple nuclear factors interact with the immunoglobulin enhancer sequeuces[J].Cell,1986,46(5):705-716.
  • 10Wang CY,Mayo MW,Baldwin AS Jr.TNF-α and cancer therapy-induced apoptosis:potentiation by inhibition of NF-κB[J].Science,1996,274(5288):784-787.

引证文献6

二级引证文献10

生物技术通讯
2006年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部