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B型肉毒毒素重链抗原表位的预测及表位合成肽抗原性的检测 被引量：1

Prediction Synthesis and Characteristic of B-Cell Epitope of Botulinum Neurotoxins Type B
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摘要 目的:预测B型肉毒毒素蛋白抗原表位,研究表位合成肽的生物学活性。方法:利用生物信息学,结合Anthwine分析软件及网络平台,预测B型肉毒毒素蛋白重链的B细胞抗原表位。人工合成4条(P1,P2,P3,P4)表位多肽,与抗B型肉毒毒素类毒素的马血清反应;采用腹腔注射的方式用合成肽免疫BALB/c小鼠,检测免疫血清与合成肽的结合;对免疫小鼠腹腔注射B型肉毒毒素。结果:合成的多肽能与抗B型肉毒毒素的类毒素的马血清结合;多肽免疫小鼠后能产生针对多肽的抗体,其中P1、P3、P4产生的抗体对受毒素攻击的小鼠具有保护作用。结论:成功预测了抗原表位,合成的表位多肽具有较好的抗原性。 Objective： To predict the B-cell epitope of BoNT type B and study the biology activity of the synthetic epitope peptide. Methods： According to the bioinformatics, combined with bio-software Anthwine and net protocol, B-cell epitope were predicted, four epitope peptides P1, P2, P3, P4 were synthesized, and their reactions with horse antiserum against BoNT toxiod were detected. Mice immunized with the peptides were challenged with BoNT/B. Results： The four peptides showed combination activity with horse antiserum against BoNT toxiod. High titers of specific antibody against the peptide antigen were induced, antibodies against P1, P3, P4 showed protection activity. Conclusion： The epitopes were predicted. The predicted epitope peptides showed high antigenicity.
作者 孟露露 荫俊 王慧 史晶 侯晓军
机构地区 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
出处 《生物技术通讯》 CAS 2006年第2期183-185,共3页 Letters in Biotechnology
基金 国家重点基础研究发展规划项目(2002CB513205)
关键词 肉毒毒素 表位 合成肽 botulinum neurotoxins epitopes synthetic peptide
分类号 Q789 [生物学—分子生物学] R392.1 [医药卫生—免疫学]
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生物技术通讯
2006年 第2期

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