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基因工程重组蛋白胞外分泌Ⅰ型系统的研究进展 被引量：1

Advance on TypeⅠ Secretion System Used as A Recombinant Protein Export Vehicle
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摘要 在基因工程领域,重组蛋白表达后定位于胞内或周质间隙,影响了其表达水平与可溶性,进而影响活性,降低了利用效率。分泌表达至胞外培养基则能较好地解决这一难题。利用细菌天然分泌系统作为重组蛋白胞外递送工具已日益成为生物工程及相关领域的常用策略,其中Ⅰ型分泌系统是研究最多、最具应用前景的。本文简要综述典型的大肠杆菌α-溶血素(HlyA)分泌系统以及更具优势的新月柄杆菌SLP(RsaA)分泌系统的生化特性、遗传特征及移植利用等方面的研究进展。 In most cases, the intracellular production of recombinant proteins by the gram-negative bacteria such as E. coli has several disadvantages, including relatively low level of expression and solubility, low biological activity and low efficiency, while secretion of heterologous proteins to the culture medium has several advantages over intracellular production. Bacterial secretion systems, especially type Ⅰ secretion system, are attractive candidates as effective veichle for exporting heterologous proteins. Both α-hemolysin （HlyA） secretion system of E.coli and RsaA secretion system of Caulobacter crescentus belong to type Ⅰ secretion system. In this paper, genetic and biochemistry characteristic of both HlyA and RsaA secretion system as well as advance on naturalization and functional maintenance of both systems in heterologous host were summarized.
作者 宁亚蕾 邹全明
机构地区 第三军医大学医学检验系临床微生物与免疫教研室
出处 《生物技术通讯》 CAS 2006年第2期235-237,共3页 Letters in Biotechnology
关键词 重组蛋白 胞外分泌 Ⅰ型分泌系统 HlyA分泌系统 RsaA分泌系统 recombinant protein extracellular secretion type Ⅰ secretion system α-hemolysin secretion system RsaA secretion system
分类号 Q51 [生物学—生物化学] Q781 [生物学—分子生物学]
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参考文献25

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共引文献8

同被引文献14

  • 1周立雄,张兆山.大肠杆菌α-溶血素分泌系统基因表达调控研究进展[J].生物技术通讯,2004,15(4):374-378. 被引量：9
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生物技术通讯
2006年 第2期

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