丙型肝炎病毒细胞培养系统研究进展

Advances of Cell Culture Systems of Hepatitis C Virus

由于缺少丙型肝炎病毒(HCV)细胞培养系统,因此对其生活周期、感染机制至今仍不十分清楚,严重阻碍了相关治疗药物的研制。HCV复制子仅能在Huh7等极少数细胞中短暂复制且量低。1999年建立了亚基因组复制子,使人们有机会对其进行深入研究,但须人为引入碱基突变。最近建立的全基因组复制子无须引入突变且可形成病毒粒子,是一项重大突破。本文概述了HCV细胞培养系统的研究进展。

The hepatitis c virus（HCV） life cycle and the mechanism of viral infection are poorly understood in the absence of efficient cell culture systems. The development of HCV-selective antiviral drugs are also hampered. HCV can propagate only in a few kinds of cell lines such as Huh7 for a short period of time. The replicon system comprising adaptive mutations in 1999 gave people the chance to research further. A great advance has been achieved recently by a robust full-length replicon without adaptive mutations, which could produce infectious HCV. In this article, the concise overview of currently available cell culture systems of HCV were provided.