中国健康人群人类白细胞抗原B27亚型频率分布

Frequencies Distribution of Human Leukocyte Antigen-B27 Subtypes in Healthy Chinese

目的调查中国健康人群中人类白细胞抗原(HLA)B27亚型的频率分布状况。方法应用聚合酶链反应—直接测序分型法对825名健康无关个体进行HLA高分辨分型。结果有25名个体呈现HLA-B27阳性(包括24名B27杂合子和1名B27纯合子个体),检出的8种亚型及相应频率分别为:B*2704(30·77%)、B*2705(23·08%)、B*2707(19·23%)、B*2711(7·69%)、B*2712(7·69%)、B*2701(3·85%)、B*2713(3·85%)和B*2721(3·85%)。结论用直接测序法获得了中国健康人群中HLA-B27亚型频率分布状况,为HLA-B27与强直性脊柱炎等疾病的相关性研究提供重要参考数据。

Objective To investigate the frequencies distribution of human leukocyte antigen （HLA） - B27 subtypes in unrelated healthy Chinese. Method Polymerase chain reaction sequence-based typing （ PCRSBT） was used to determine HLA high-resolution genotypes of 825 unrelated healthy Chinese. Results A total of 25 HLA-B27-positive individuals and 8 HLA-B27 subtypes were detected. These subtypes and their corresponding frequencies were B ＊ 2704 （30. 77% ）, B ＊ 2705 （23.08%） , B ＊ 2707 （ 19.23% ）, B ＊ 2711 （7.69%）, B ＊ 2712 （7.69%）, B ＊2701 （3.85%）, B ＊ 2713 （3.85%） and B ＊ 2721 （ 3.85% ）. Conclusion The data obtained through PCR-SBT method may serve as important reference for the research of relationship between HLA-B27 subtypes and some diseases such as ankylosing spondylitis.