肝细胞癌中脆性组氨酸三联体基因的甲基化状况及其临床意义

Clinicopathological significance of aberrant methylation of the fragile histidine triad gene in patients with hepatocellular carcinoma

目的了解肝细胞癌(HCC)中脆性组氨酸三联体(FHIT)基因的甲基化状况及其临床病理意义。方法应用甲基化特异性PCR(MSP)技术,检测45例HCC癌组织及其相应的癌旁组织、14例正常肝组织和4例HCC细胞株中FHIT基因的甲基化状况,并分析其与临床参数之间的关系。结果FHIT基因在HCC癌组织、癌旁组织、正常肝组织及HCC细胞株中的甲基化率分别是71.1%、64.4%、14.3%和75.0%。FHIT基因异常甲基化组与非甲基化组在术后1年无瘤生存率比较中有统计学差异(P=0.0430)。结论HCC中FHIT基因启动子甲基化是一种普遍的早期事件,可能预示着HCC患者预后较差。

Objective To investigate the aberrant methylation of fragile histidine triad （FHIT） gene and to explore possible relationship between the aberrant methylation of FHIT and clinicopathological features in hepatocellular carcinoma （HCC）. Methods The hypermethylation of FHIT was detected by the methylation specific PCR （MSP） method in 45 patients with HCC （ tumoral and nontumoral tissue）, 14 cases of normal livers and 4 HCC cell lines （SK-Hep-1, Hep-G2, Hep-3B and Huh7）. The correlation of FHIT methylaion and clinicopathological features was analyzed. Results The frequencies of hypermethylation of FHIT in tumoral and nontumoral tissue, normal liver and cell lines were 71.1%, 64. 4%, 14. 3% and 75.0%, respectively. A significant relation between hypermethylation of FHIT and poor survival was present （ P = 0. 0430 ）. Conclusions Hypermethylation of FHIT is a frequent and early event in HCC, it might relate to a poor prognosis for patients with HCC.