过氯酸铵致肺纤维化作用的研究

The effects of ammonium perchlorate (AP) on pulmonary fibrosis

目的研究过氯酸铵(AP)致肺纤维化作用。方法 AP染毒大鼠肺泡巨噬细胞(AM)24 h, 取AM上清液培养肺成纤维细胞(FB),检测AM转化生长因子-β1(TGF-β1)蛋白表达水平及FB增殖活性、细胞内羟脯氨酸(Hyp)含量。采用气管内注入方式对大鼠进行AP染毒,3 d后处死,测定肺组织中TGF- β1 mRNA的表达。结果 AP低、高剂量组TGF-β1细胞阳性率和AP高剂量组TGF-β1光密度值均显著高于对照组(P<0．01)。各AP染毒组FB增殖活性和FB Hyp含量与对照组比较,差异无显著性(P>0．05)。AP 低、中、高剂量组肺组织TGF-β1mRNA表达水平均高于对照组(P<0．05或P<0．01)。结论 AP增加了 AM和肺组织TGF-β1表达,但尚未表现出致肺纤维化作用。

Objective To explore the effects of AP on pulmonary fibrosis. Methods Alveolar macrophages （AM） were treated by AP for 24 hours. Pulmonary fibroblasts （FB） were cultured with the supematant of AM medium. The protein of transforming growth factor beta 1 （TGF-β1） of AM, the proliferative activity and hydroxyproline （Hyp） content of FB were examined. Rats were treated with AP by intratraeheal instillation and sacrificed at 3 days. The TGF-β1 mRNA content in the lung was examined. Results The positive staining maerophages in low and high AP groups and the quantity of TGF-β1 in high AP group were obviously higher than those in the control group （ P 〈 0.01 ）. But there was no significant difference in the proliferative activity and Hyp content of FB in all groups. The mRNA contents of TGF-β1 in three AP groups were all significantly higher than that in the control group （ P 〈 0.05 or P 〈 0.01 ）. Conclusion AP increased the expression of TGF-β1 in AM and lung, but pulmonary fibrosis was not observed.