摘要
目的探讨大鼠空肠上皮细胞株(IEC-6)受到不同剂量γ射线照射后,ERp29蛋白(endoplasmic reticulum protein)在不同时相点的表达变化情况。方法分别提取正常IEC-6细胞和5、101、52、0 Gy照射后3、12、24、48、72 h细胞的蛋白样品,采用Western blotting方法检测ERp29的表达。结果不同剂量照射后IEC-6 ERp29蛋白表达比正常细胞高,蛋白表达随照射时间的增加而增加。结论电离辐射可诱导IEC-6细胞高表达ERp29蛋白,提示该蛋白在肠道的损伤修复中起重要作用。
Objective To analyze the change of ERp29 protein in IEC-6 cells irradiated by γ-rays. Methods The total protein was isolate t from the normal IEC-6 cells and the IEC-6 cells irradiated by 5, 10, 15, and 20 Gy γ-rays, respechtwely. The expression of ERp29 protein was confirmed by Western blotting. Results The total proteins of normal and irradiated IEC-6 cells were successfully distilled. Expression of ERp29 in irradiated IEC-6 cells was higher than that in normal IEC-6 cells, and was increased with the prolongation of irradiation time. Conclusion γ-ray irradiation can induce high expression of ERp29 protein in intestinal epithelium cell line, which indicates ERp29 protein may play an important role in damage and repair of intestine.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2006年第3期286-288,共3页
Immunological Journal
基金
国家自然科学基金重点项目
国家自然科学基金面上项目(30230360
30400121)资助