树突细胞与转化生长因子β_1在食管癌组织中表达的研究

Expressions of dendritic cell and transforming growth factor beta-1 in esophageal cancer tissue

目的通过检测食管癌组织中树突细胞(dendrtic cell,DC)与转化生长因子1β(TGF-1β)的表达水平,探讨食管癌病人发生免疫逃逸的原因。方法采用酶标记的链霉亲和素生物素法(LSAB)对94例食管癌组织进行抗S100、抗TGF-β1表达的检测以及采用RT-PCR技术进行CD1a、TGF-β1mRNA表达的检测。结果食管癌组织分化程度越高,癌组织内S100蛋白阳性DC密度及DC的标记分子CD1a mRNA的表达水平越高(P=0.026,0.000 1);肿瘤分化程度越低,TGF-1β蛋白及mRNA的表达水平达越显著(P=0.041,0.000 1)。TGF-β1蛋白及mRNA的表达越显著,癌组织内S100蛋白阳性DC的密度及DC的标记分子CD1a mRNA的表达越低(P=0.024,0.000 1)。结论TGF-1β抑制DC表面分子CD1a mRNA的表达,影响DC的分化与成熟,使肿瘤组织中参与抗肿瘤免疫的DC数量减少,进而抑制机体的免疫系统,是肿瘤细胞逃逸免疫系统监视的重要原因之一。

Objective To discusses the expressions of dendritic cell （DC） and transforming growth factor beta- 1 （TGF-β1） in esophageal cancer tissue and explore the mechanism of immune escape of the esophageal cancer cell. Methods S100 protein and TGF-β1, were detected by labeled streptavidin biotin （LSAB） immunohistochemical method. The expressions of CDla and TGF-β1 mRNA in 94 patients with esophageal cancer were detected by reverse transcription polymerase chain reaction （PT-PCR）. Results The number of S100^＋ protein dendritic cell and the mRNA expression of CD1a molecules of the DCs were positively correlated with the pathological differentiation （ P = 0.026, 0.000 1）. The worse the pathological differentiation was, the more TGF-β1 protein and the mRNA expression were （ P = 0.041, 0.000 1）. The more the TGF-β1 protein and the mRNA expression were, the less the S100^＋ protein dendritic cell and the mRNA expression of CD1a molecules of the DCs were （P = 0.024, 0.0001）. Conclusion TGF-β1, inhibits the mBNA expression of CD1a molecules of DCs, andimpairs the differentiation and mature of the DCs, and influencs the expression of DCs, which is one of the reasons for cancer cell escape from the immunity of the body.