移植性人白血病小鼠模型的建立

Establishment of human leukemia model by transplanting K562 cells into SCID mice

目的:建立移植性人白血病重度联合免疫缺陷(SCID)小鼠模型,探讨人白血病细胞在SCID小鼠体内的生物学行为,为研究药物等因素治疗白血病的体内方法奠定基础。方法:在SCID小鼠腹腔或静脉分别接种5×106～1×107个K562细胞,应用组织细胞化学、RT-PCR、流式细胞术、组织病理监测SCID小鼠的外周血、骨髓、肝脏、脾脏的白血病细胞。结果:K562细胞经静脉或腹腔途径均可植入SCID小鼠体内,形成白血病模型;3w时外周血即可见白血病细胞,肝、脾脏增生期细胞达40.11%、41.66%;发病晚期,模型动物骨髓细胞中CD13+细胞占10.15%;接种K562细胞后能检测到人DQα,ABL-BCR基因,接种K562细胞后3～4w即可见白血病细胞累及肝、脾、肾、骨髓等主要脏器。结论:SCID小鼠接种K562白血病细胞能成功建立白血病动物模型,CD13阳性率能快速、客观地判断模型中肿瘤细胞浸润程度。

Objective： To establish the transplanted leukemia severe combined immunodeficiency （SCID） mouse model and to explore the biological action of human leukemic cells in SCID mice. Methods： 5 × 10^6 - 1 × 10^7 K562 cells were inoculated into the abdominal cavity or vein of SCID mice. Immunocytochemistry, RT-PCR, flow cytometry and histopathology were used to detect the leukemic cells in the peripheral blood, bone marrow, liver and spleen of SCID mice. Results： K562 cells could be transplanted into SCID mice through abdominal cavity or vein, and leukemia animal model was successfully established. 3 weeks after transplantation, the leukemia cells could be found in the peripheral blood, The proliferation phase cells in the liver and spleen reached 40.11% and 41.66% respectively. In the terminal stage, the CD13^＋ cells accounted for 10.15% of bone marrow cells in model animal. The DQ α and ABL-BCR genes could be detected after injection of K562 cells, and 3 to 4 weeks after injection, the leukemic cells spread to the liver, spleen, kidney, bone marrow, and so on. Conclusion： The leukemia animal model could be successfully established by transplanting K562 cells into SCID mice. Positive rate of CD13 might be used as the index of tumor cells infiltration.