人羊膜上皮细胞在纤维蛋白支架和细胞生长因子作用下的研究

Study of the human amniotic epithelial cells on the fibrin clot and cellular growth factor

目的:以纤维蛋白凝块为支架构建人羊膜上皮细胞(HAEC)生长模型,观察表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)、转化生长因子β1(TGF-b1)对HAEC生长的影响。方法:在体外构建的纤维蛋白支架上培养HAEC,采用倒置显微镜、Giemsa染色和扫描电子显微镜观察HAEC的生长情况。采用TUNEL法检测EGF、bFGF和TGF-β1对纤维蛋白支架上HAEC凋亡的影响。结果:HAEC在构建的纤维蛋白支架上生长。EGF组和bFGF组HAEC凋亡较对照组明显减少(P<0.05),TGF-β1组HAEC凋亡较对照组明显增加(P<0.05)。结论:HAEC能在构建的纤维蛋白支架上良好生长,EGF、bFGF可抑制HAEC凋亡,TGF-β1则促进HAEC凋亡。

Objective： To establish the model of cultured human amniotic epithelial cells （HAECs） on the fibrin clot in order to observe the effect of EGF, bFGF and TGF- β1 on HAEC. Methods ：The cultivated HAECs on the formulated fibrin clot were examined by phase contrast microscopy, Giemsa staining and scanning electron microscopy. The effect of growth factor （EGF, bFGF, and TGF- β1） on the apoptosis of HAECs was measured by TUNEL. Results： HAECs generated on the formulated fibrin clot in vitro. HAECs exposed to EGF or bFGF showed a lower rate of apoptosis than the control group （P 〈 0.05）, however, HAECs exposed to TGF- β1 showed an opposite effect （P 〈 0.05）. Conclusion： HAECs generate well on the formulated fibrin clot in vitro. Exposure to EGF or bFGF decreases the apoptosis rate of HAEC, however, exposure to TGF-β1 increases the apoptosis.