孕鼠肝内胆汁淤积症致胎鼠心律失常的实验研究

Fetal rats arrhythmias in intrahepatic cholestasis of pregnancy

目的:探讨孕鼠肝内胆汁淤积症致胎鼠心电图的异常。方法:应用雌、孕激素建立妊娠肝内胆汁淤积症大鼠模型,通过Powerlab生物信号处理系统Chart 5软件引导妊娠第20天和第21天胎鼠宫内心电图。结果:(1)妊娠第20天,胆淤模型组1孕鼠血清总胆汁酸(TBA)水平为(64.3±5.2)μmol/L;妊娠第21天,胆淤模型组2孕鼠血清总胆汁酸(TBA)水平为(78.5±4.5)μmol/L。两组比较差异有极显著性,P<0.01。(2)妊娠第20天,胆淤模型组1持续观察90min,胎鼠心电图均显示正常;妊娠第21天,胆淤模型组2实验开始时胎鼠心电图正常,持续观察29.3±6.4min后出现胎鼠心律不齐,继续观察23.5±4.6min后胎鼠死亡,而正常对照组2持续观察90min,胎鼠心电图均正常。结论:孕鼠肝内胆汁淤积症时胎鼠死亡前出现短暂的心律失常阶段。

Objective： To investigate fetal rats abnormal electrocardiograms in intrahepatic cholestasis of pregnancy （ICP）. Methods： The animal model of intrahepatic cholestasis of pregnancy was induced by 17-α-Ethinylestradiol and Progesterone. The electrocardiograms of fetal rats by conceptual day 20^th （Groupl）, day 21^st （Group2） were evaluated with Powerlab biologic signal extracting and analyzing system Chart 5 Software. Resu/ts： ①The serum total bile acids （TBA） levels of pregnant rats of ICP were 64.3 ± 5.2（μ mol/L） in group 1.78.5 ± 4.5（μmol/L） in group 2, respectively, which showed significant differences （P〈0.01）. ②It showed all normal electrocardiograms of fetal rats in Groupl over 90 minutes continuous observation. In Group2, fetal rats arrhythmias appeared after 29.3 ± 6.4 minutes normal electrocardiograms observed initially; About 23.5 ± 4.6 （ range 15-30 ） minutes fetal rats arrhythmias later, fatal ventricular arrhythmias came out and the fetal rats died suddenly. Conclusion： It demonstrated that fetal arrhythmias contribute to fetal sudden death in ICP.