慢性痛大鼠背根神经元基因表达的研究

Study on Gene Expression of Neurons in Dorsal Root Ganglia of Rat with Neuropathic Pain

目的研究与慢性痛相关基因的特异表达,比较大鼠背根神经节损伤神经元与正常神经元之间基因表达的差异,以寻找构成神经病性疼痛的内在因素。方法应用mRNA差异显示方法从损伤背根神经节中寻找特异表达的基因。结果损伤侧背根节中cDNA条带(25.75±4.7)明显多于对照侧(18.0±5.0)。反向杂交后进行亚克隆得到10个含插入片段的阳性质粒,并对其中4个进行测序。结论神经轴突损伤可导致胞体基因表达改变,其中某些可能与痛觉异常有关,也可能与细胞结构恢复及免疫功能改变有关。

Objective To study the specific expression gene related chronic pain and to investigate on intrinsic reason of neuropathic pain, gene expressions were compared between normal neurons and injured ones of DRGs from rats. Methods mRNA differential display was used in searching for specific expression gene from injured dorsal root ganglia neurons of chronic constriction injury （CCI） rats. Results It was found that cDNA bands were significantly increased in CCI DRGs （25.7 ± 5 4. 7） than in normal ones （18.0 ±5.0）. 10 plasmas with inserted genes were obtained from subclones after inverse hybridization, and 4 of them were sequenced. Conclusions It has been proved that axon injury of the neuron can result in alteration in genes expression of the cell soma, some of which may be involved in allodynia, others may be related to cell＇s structure reconstruction or change in immunological functions.