肝纤愈对免疫性肝纤维化大鼠TNF-α、IL-1水平的影响

Effect of GanXianYu Compound on the Level of the TNF-α,IL-1 of Immunity Liver Fibrosis in Rats

目的建立大鼠免疫损伤性肝纤维化的实验模型,研究中药复方肝纤愈对细胞因子TNF-α、IL-1含量的影响,探讨其抗肝纤维化的作用机制。方法在以人血白蛋白(HSA)诱导的免疫损伤性肝纤维化大鼠模型基础上将肝纤愈分为小剂量(6.1 g/kg)组,大剂量(12.2 g/kg)组灌胃给药,连续8周,以鳖甲软肝片和秋水仙碱为阳性对照组;以放射性免疫方法测定大鼠血清中TNF-α、IL-1含量。结果肝纤愈大剂量组可明显降低HSA诱导免疫性肝纤维化大鼠模型血清中TNF-α、IL-1的水平。结论认为肝纤愈的作用系对TNF-αI、L-1等细胞因子的影响而产生抗肝纤维化作用。

Objective To explore mechanism of anti-fibrosis ,the rat model with immunity liver fibrosis was established to study the effect of compound herbal medicines of GanXianYu on the levels of TNF-α, IL-1. Methods The rat models with immunity liver fibrosis were induced by the human serum albumin, GanXianYu were taken by gastric infusion respectively as little and large dosage group （6. 1g·kg^-1 and 12. 2g·kg^-1 ） taken consecutive for 8 weeks. Biejia Ruangan Tablet or colchicines were taken as positive contrast drug. The levels of TN-α,IL-1 were detected by radioimmunoassay. Results GanXianYu could depress the serum levels of 1 TNF-α, IL-1 in rat models with immunity liver fibrosis induced by the human serum albumin in large dosage groups. Conclusion GanXianYu could treat liver fibrosis through affect the lever of TNF-α,IL-1 in liver.