南方红豆杉炭疽病病原鉴定及其生物学特性

The identification and biological characteristics of the anthracnose pathogen of Southern Chinese yew

对福建明溪南方红豆杉苗木炭疽菌进行分离鉴定,结果表明病原菌为胶孢炭疽菌[Col-letotrichum gloeosporioides(Penz.)Sacc.].病原菌菌丝在查氏培养基上生长最好;病原菌菌丝生长的最适温度是25℃,最适pH值为7;产孢的最适温度为30℃,最适pH值为8;光暗交替最有利于菌丝生长,自然散光最有利于产孢;对碳源的利用,葡萄糖最有利于菌丝生长,蔗糖最有利于孢子的形成;对氮源的利用,蛋白胨最有利于菌丝生长,牛肉浸膏最有利于孢子的形成;孢子萌发的最适温度是25℃,最适pH值为6;RH在70%-100%均能萌发,在水滴中萌发率最高,为76.18%;RH低于60%不萌发;光照有利于孢子的萌发.

The results of the identification and biological characteristics research of Colletotrichum spp. on Southern Chinese yew in Mingxi of Fujian proved to be Colletotrichum gloeosporioides （ Penz. ） Sate. The mycelia grew best on the Czapek＇Agar; The optimum temperature and pH for hypha growth was 25℃ and 7, respectively while those for conidium formation was 30 ℃ and 8 ; The alternation of light and darkness every 12 hours was found to be conducive to the hypha growth and the natural light to the conidium formation. The optimum carbon source and nitrogen source for hypha growth was glucose and protein peptone, respectively while those for eonidium formation was sucrose and beef extracts; The optimum temperature and pH for the conidium germination was 25℃ and 6, respectively; The conidium could germinate in relative humidity 70% - 100% or in water, but the conidium has high germinate rate （76. 18% ） only in drops of water, The conidium couldnt germinate at lower than 60% RH; The light was beneficial for the eonidium germination.