几种野生稻甲醇提取物对害虫的行为干扰及对酯酶同工酶的影响

Deterrent Activity of Extracts from Several Species of Wild Rice to Panonychus citri and Aphis citricola in Associated with Esterase Isoenzyme in Pests

采用索氏回流法,经甲醇提取,获得大颖野生稻(E6-3/6-4、E6-1)、小粒野生稻(E13-9、E13-13)、药用野生稻(E15-8、E15-13)、斑点野生稻(E16-1、E16-3、E16-13)、阔叶野生稻(101392、E9-1、E9-10)和颗粒野生稻(E7-4)甲醇提取物。室内生物活性测定表明这些野生稻甲醇提取物对柑橘全爪螨和绣线菊蚜具有一定的忌避活性,其中药用野生稻E15-8的甲醇抽提物效果更为显著,5×104μg/mL的浓度处理,测得24h和48h对柑橘全爪螨的忌避率分别为83.26%和87.95%;用同样的浓度处理,对绣线菊蚜的忌避率分别为87.86%和82.43%。经聚丙烯酰胺凝胶电泳检测,药用野生稻E15-8的甲醇抽提物对两种害虫的酯酶同工酶具有明显的抑制效果。

Six species of wild rice and their different ecotypes including Oryza grandiglumis （E6-1, E6-3/6-4）, O. minuta （E13-9, E13-13）, O. officinalis （E15-8, E15-13）, （9. punctata（E16-1, E16-3, E16-13）, O. grartulata（ET-4）, O. latifolia （101392, Eg-1, Eg-10） were extracted with methanol. Deterrent activity of the extracts to Aphis citricola and Panonychus cirri was tested. The extract from the wild rice O. officinalis E15-8 was better than the others in the deterrent activity to the two pests. The deterrent rates of E15-8 to Panonychus cirri and Aphis citricola were 83. 26% and 87. 86% in 24 h and 87.95 % and 82.43 % in 48 h at 5 × 10^4μg/mL concentration, respectively. The methanol extracts from O. officinalis E15-8 had the effect of inhibition to the esterase of the two pests.