摘要
研究了茜素红S作为光散射探针测定蛋白质的分析方法.在pH=4.0的Britton-Robinson缓冲溶液中。茜素红S与蛋白质结合后有强烈的光散射作用,在λ=353nm处,光散射强度最大,且光散射强度与蛋白质浓度成正比.由此建立了测定蛋白质含量的分析方法.方法灵敏度高,检测限达0.01mg/L,线性范围为0.02~6mg/L.方法适用于人血清蛋白含量的测定.
This article proposed an analytical method for determining the content of proteins, using alizarin red S(ARS) as the resonance light scattering probe. In Britton-Robinson buffer at pH 4. 0, ARS combined with proteins by intermolecular forces, causing the enhancement of resonance light scattering (RLS) at maximum wavelength of about 353nm, and the RLS intensity is proportional to the concentration of proteins. The assay is simple, sensitive with a detetion limit of 0. 01mg/L and a linear ranging up 0.02 ~ 6 mg/L. The method had been used to determine the total proteins in human serum samples.
出处
《中南民族大学学报(自然科学版)》
CAS
2006年第1期12-14,共3页
Journal of South-Central University for Nationalities:Natural Science Edition
基金
湖北省自然科学基金资助项目(2005ABA001)
中南民族大学自然科学基金重点资助项目(YZZ03003)
关键词
茜素红S
蛋白质
光散射
Alizarin Red S
protein
light scattering