茜素红S与蛋白质作用的光散射光谱及其分析应用

Determination of Protein with Alizarin Red S as a Probe of Light Scattering

研究了茜素红S作为光散射探针测定蛋白质的分析方法．在pH=4．0的Britton-Robinson缓冲溶液中。茜素红S与蛋白质结合后有强烈的光散射作用，在λ=353nm处，光散射强度最大，且光散射强度与蛋白质浓度成正比．由此建立了测定蛋白质含量的分析方法．方法灵敏度高，检测限达0．01mg／L，线性范围为0．02～6mg／L．方法适用于人血清蛋白含量的测定．

This article proposed an analytical method for determining the content of proteins, using alizarin red S（ARS） as the resonance light scattering probe. In Britton-Robinson buffer at pH 4. 0, ARS combined with proteins by intermolecular forces, causing the enhancement of resonance light scattering （RLS） at maximum wavelength of about 353nm, and the RLS intensity is proportional to the concentration of proteins. The assay is simple, sensitive with a detetion limit of 0. 01mg/L and a linear ranging up 0.02 ～ 6 mg/L. The method had been used to determine the total proteins in human serum samples.