针刺小肠经干预急性心肌缺血大鼠心脏基因表达谱研究

The Study on Effect of the Heart Merdian of the Small Intestine Meridian of Hand- Taiyang on Gene Experession Pattern of Heart in Rats with Acute Myocardial Ischemia

目的从基因水平揭示小肠经干预心肌缺血作用分子心脏学机制。方法随机将SD大鼠分为模型组和小肠经组,采用结扎冠状动脉前降支法复制急性心肌梗死动物模型,电针手太阳小肠经“养老(SI5)-支正(SI7)”段。对两组动物心脏基因表达谱变化进行比较分析。结果与模型组比较,小肠经组共有848个差异表达基因(包括表达序列标签),其中347个表达下调,501个表达上调,表达差异大于2倍的分别有26个和18个。小肠经组差异表达大于2倍的基因主要是离子通道和运输蛋白相关基因,代谢相关基因,细胞信号和传递蛋白相关基因,DNA结合、转录和转录因子类基因,免疫和炎性反应相关基因等。结论电针小肠经可能是通过影响上述基因,从而对急性心肌缺血发挥保护作用。

Objective:To find out heart molecular mechanism of effect of the Small Intertine Meridian of Hand Taiyang in protecting against acute myocardial ischemia in gene level.Methods:Ten Sprague- Dawley(SD) rats were randomly divided into model group and Small Intestine Meridian group,each group had 5 rats.A model of acute myocardial ischemia (AMI) was made by ligation of left anterior descending branch of the coronary artery.The meridian sections of ' Yanglao (SI5)- Zhizhen(SI7)' of the Small Intestine Meridian of Hand Taiyang was stimulated with multi- electroacupuncture.A comparative study on changes of gene expression pattern of Small Intestine Meridian group vs model group in heart was done to find common and differential expression genes.Results:Up- and- down- regulated genes,including expressed sequence tags (ESTs),were 18 with signal log ratio≥ 1 and 26 with signal log ratio≤ - 1, of 501 and 347 in Small Intestine Meridian group vs model proup.They mainly included some Ion meridianand transport protein relative genes,metabolism relative genes,cell signal transduction and transferin relative genes,DNA's binding,transcription and transcriptional factor relative genes,immumological and inflammatory reaction relative genes,et al.Conclusion:The Small Intestine Meridian may play a role in protecting against acute myocardial ischemia with the above related- genes.