十全大补汤诱导肝癌H_(22)小鼠肿瘤细胞凋亡实验研究

The Experimental Study on Shiquandabu Decoction (十全大补汤 ) Inducing Tumor Cell of Liver Cancer H_(22) Mouse to Weakly Perish

目的探讨十全大补汤对小鼠肝癌H22抑瘤作用及其机理。方法复制小鼠肝癌H22模型,实验分为模型组,5-FU组,中药高、中、低剂量组,分别计算抑瘤率;用免疫组化法检测凋亡指数。结果中药高、中、低剂量组均有一定的抑制作用,以中剂量组为最佳,抑瘤率为54.8%,且增加凋亡指数;与5-FU组比较,均差异显著。结论中药(十全大补汤)高、中、低剂量组对小鼠肝癌H22有明显抑瘤作用,以中剂量组为最佳,增加凋亡指数可能是其抑制肿瘤的主要机理之一。

Objective:To observe Shiquandabu Decoction (十 全 大 补 汤 )'s function of suppressing lumps and its mechanism to liver cancer H22 mouse.Methods:Duplicate mouse liver cancer H22 model.the experiment is divided into the model group,the 5- Fu group,Shiquandabu Decoction in high- dose group,middle- dose group and small- dose group,calculates separately the rate of suppressing lumps;Examining the indes of perishing with the immunohistochemistry techniques.Results:Shiquandabu Decoction in high- dose group,middle- dose group and small- dose group all have definite function of suppressing lumps.The middle- dose group is as the best,whose suppressing lumps rate is 54.8% ,and increases the index of perishing;Compared with the 5- FU group,the three groups' differences are all remarkable.Conclusion:Shiquandabu Decoction in high- dose group,middle- dose group and small- dose group have obvious function of suppressing lumps to liver cancer H22 mouse,the middle- dose group is as the best.Increasing the index of perishing may be one of its main mechanisms of suppressing tumor.