摘要
为了研究家兔睾丸间质细胞(Leydigcell,LC)的分离纯化程序及体外生长特点,试验采用4种不同消化程序对家兔睾丸组织进行消化,percoll梯度分离出兔LC,并进行体外培养。结果表明:经4℃保存和34℃孵育,0.5mg/mLⅠ型胶原酶消化40min获得的细胞数最多,经percoll梯度分离的细胞纯度达92%;34℃、5%CO2条件下细胞生长良好,呈现梭形和不规则形2种形状。
In order to research on the process of separating leydig cells and the character of Leydig cells cultured in vitro,rabbit testises were digested in four digesting methods,and leydig cells were purified by percoll separation and cultured in vitro. The results show： the best process is 4℃ keep, 34℃ incubation, and digestion by 0.5 mg/mL collagenase type 1 for 40 mins; the rate of LC can reach 92% using percoll separation, and shuttle - like and irregular cells growed in vitro in 34℃ ,5% CO2
出处
《黑龙江畜牧兽医》
CAS
北大核心
2006年第5期12-14,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
转基因动物生产基因工程药物研究(D08)
