金鱼视觉系统内中、高分子量神经丝免疫组织化学定位

Immunohistochemical localization of the medium and high-weight neurofilament in the visual nervous system of Carassius auratus

RT97是一种特异性识别中、高分子量神经丝蛋白抗原簇的抗体.采用组织化学技术对金鱼(Carassiusauratus)视觉系统内RT97识别神经丝的表达分布进行了观察.结果表明,RT97在金鱼视网膜、视神经和视顶盖中均有阳性反应:①在视网膜内视纤维层、内网层、内核层的水平细胞、光感受器的外节有RT97阳性反应;②在视乳突部和视神经有少量生长纤维为RT97阳性;③在视顶盖中RT97阳性反应集中在顶盖的边缘层(SM),同时观察到有RT97阳性纤维从中央纵突区(TL)通过顶盖腹面延伸至顶盖边缘区.实验结果提示,RT97不仅可作为金鱼视觉系统视觉生长纤维的一种标记蛋白,同时在视顶盖由RT97识别的蛋白也可能是一种吸引视觉纤维生长的靶蛋白.

RT97 is known to be specific for high and medium-weight neurofilament subunits in mammals. RT-97 immunoreactivity in the visual nervous system of goldfish is studied by the immunochemical method. Our results demonstrate that the positive RT-97 immunoreactivity can be observed in the OFL, IPL, the horizontal cell of INL and the outer segment of the photoreceptive cells, a few of growing fibers can be observed to be positive in the optic nerve head and optic nerve. The RT-97 immunoreactivity mainly accumulate in the SM. And the positive RT-97 fibers can be observed to originate from the TL and go through the dorsal layers of the tectum to the SM. Our results indicate that RT-97 can be one good marker to illustrate the growing fibers and also one target candidate molecular in the tectal target to attract the retina fibers.