摘要
目的探讨人肝细胞体内移植治疗肝衰竭患者的有效性与安全性。方法分离健康志愿者捐献的肝脏,获得人原代肝细胞并冷冻保存,复苏后经股动脉插管移植到脾脏,观察治疗前后肝衰竭患者临床症状、血液生化指标及脾脏核磁共振信号的改变。结果人肝脏可获取2×1010肝细胞,复苏后肝细胞活率在70%以上,移植的肝细胞数为2×109个。移植后1个月,患者临床症状明显改善,血胆红素、NH3、转氨酶明显降低,凝血酶原活动度水平明显升高。出院50d后随访各项血生化指标均恢复正常,脾脏内可见肝细胞信号。结论肝细胞体内移植是一项安全有效的治疗方法,移植的肝细胞能在脾脏内增殖、分化,替代或部分恢复肝脏合成、解毒和代谢功能,将为终末期肝病治疗开辟一项新的治疗措施。
Objective To evaluate the efficacy and safety of human hepatocyte transplantation in treatment of patients with hepatic failure. Methods Hepatocytes were isolated from the healthy donator liver and then cryopreserved. The rewarmed hepatocytes were transplanted in to the patient＇ s spleen with transcutaneous femoral artery catheter ization, Results 2×10^10 hepatocytes were hatred and viability of rewarmed hepatocytes was 70% . One month later, the symptoms of recipient were ameliorated and levels of BII,, NH3, ALT, AST reduced, PA increased. Follow-up study demonstrated that biochemlca] profiles became normal and hcpatoeyte signal was seen in the spleen by MRI,Conclusion Hepatocyte transplantation is safe and effective,
出处
《肝脏》
2006年第2期75-77,共3页
Chinese Hepatology
关键词
重型肝炎
肝细胞移植
肝衰竭
Hepatic failure
Hepatitis
Hepatocyte transplantation
