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PCR-CTPP技术在宫颈癌P73基因多态性研究中应用

Application of PCR-CTPP for P73 Polymorphism in Cervical Carcinoma
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摘要 采用PCR-CTPP技术检测分析新疆维吾尔族宫颈癌P73基因第二外显子G4C14-to-A4T14多态性,并与PCR-RFLP技术检测结果对比,探讨PCR-CTPP在SNP检测及分析中的应用。结果显示:经PCR-CTPP扩增出P73基因第二外显子G4C14-to-A4T14三种基因型相应的DNA片段,与DNA测序结果符合。说明PCR-CTPP技术成本较低、省时、操作简便,结果稳定可靠,对于SNP研究具有较高的应用价值。 Objective：to explore the application of PCR-CTPP and analysis of SNP. Methods： PCR-CTPP is used to detect polymorphism of p73 exon 2 G4C14-to-A4T14 in cervical carcinoma of Uygur women. Results： DNA bands of three different genotyp can be amplified by using PCR-CTPP, which is consistent with the result of the sequencing. Conclusion： PCR-CTPP is an inexpensive, easy time-saving and reliable method compared with other methods.
作者 郑莉莉 杨安强 潘晓琳 郑兴征 李洪安 梁伟华 郑玉琴
机构地区 新疆地方与民族高发病实验室 石河子大学医学院 石河子大学医学院第一附属院
出处 《石河子大学学报（自然科学版）》 CAS 2006年第1期41-44,共4页 Journal of Shihezi University(Natural Science)
基金 国家自然科学基金资助项目(30260036)
关键词 PCR-CTPP P73基因多态性 宫颈癌 PCR-CTPP P73 polymorphism cervical carcinoma
分类号 R737.33 [医药卫生—肿瘤]
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参考文献6

  • 1Hamajima N,Saito T,et al.Competitive Amplification and Unspecific Amplification in Polymerase Chain Reaction with Confronting Two-Pair Primers[J].Journal of Molecular Diagnostics,2002,4(2):103-107.
  • 2Hamajima N,Saito T,et al.Polymerase Chain reaction with Confronting Two-pair Primers for Polymorphism Genotyping[J].Cancer Res,2000,91:865-868.
  • 3Craveiro R,Costa S,et al.TP73 alterations in cervical carcinoma[J].Cancer Genet Cytogenet,2004,150(2):116-121.
  • 4Niwa Y,Hamajima N,et al.Genetic polymorphisms of p73 G4C14-to-A4T14 at exon 2 and p53 Arg72Pro and the risk of cervical cancer in Japanese[J].Cancer Lett,2004,205(1):55-60.
  • 5Li G,Sturgis E M,et al.Association of a p73 exon 2 G4C14-to-A4T14 polymorphism with risk of squamous cell carcinoma of the head and neck[J].Carcinogenesis,2004,25(10):1911-1916.
  • 6周晨慧,彭惠民.单核苷酸多态性检测方法的评价[J].现代医药卫生,2005,21(2):156-157. 被引量：3

二级参考文献9

  • 1llou P, Wagener C. Methods for detection of point mutations: performance and quality assessment[J] .Chem, 1997,43: 1114.
  • 2Qiang Liu, Erik C,Thorland, et al. Heit Overlapping PCR for Bidirectional PCR Amplification of Specific Alleles: A Rapid One - Tube Method for Simultaneously Differentiating Homozygotes and Heterozygotes[J].Genome Research, 1997,7(4) :389.
  • 3Maxim V,Myakishev,Yuri Khripin,et al.High -Throughput SNP Genotyping by Allele - Specific PCR with Universal Energy - Transfer - Labeled Primers[J] .Genome Research,2001,11(1): 163.
  • 4Toumier I, Raux G, Di Fiore F, et al. Analysis of the allele- specific expression of the mismatch repair gene MLH1 using a simple DHPLC -Based Method [ J ]. Hum Mutat, 2004, 23 (4): 379.
  • 5Xiyuan Sun, Ding H, Hung K, et al.A new MALDI -TOF based mini sequencing assay for genotyping of SNPS[J] .Nucleic Acids Research,2000,28(12) :68.
  • 6Andreas Braun,Daniel P. Little, Hubert Koster. Detecting CFTR gene mutations by using primer oligo base extension and mass spectrometry[J].Clinical Chemistry, 1997,43: 1151.
  • 7Timothy J. Griffin, Jeff G. Hall, James R, et al. Direct genetic analysis by matrix - assisted laser desorption/ionization mass spectrometry[J]. Genetics, 1999,96(11) :6301.
  • 8Nader Pourmand, Elahe Elahi, Ronald W Davis, et al. Multiplex Pyrosequencing [ J ]. Nucleic Acids Research, 2002, 30 (7): 31.
  • 9Daniel Ramon,Megan Braden,Sharon Adams,et al. Pyrosequencing(tm) :A one - step method for high resolution HLA typing[J] .Transl Med,2003, 1 (1) :90.

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石河子大学学报（自然科学版）
2006年 第1期

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