摘要
胚囊观察是植物胚胎学的主要研究方法之一。我们在学习了酶解法之后,摸索研究了一种观察甜菜胚囊的压片法。其步骤:固定、硼砂洋红整体染色、酸解离、剥离胚珠、透明、压片、观察。观察到了胚囊的一些情况。此法简捷易行,成本低。
The embryo sacs observation is one of the main study method for embryology. After we learnt enzymatic isolation method of YANG Hong-yuan and ZHOU Chang, we explored to develop a pressure cover glass method of observation on embryo sacs. The procedures are fixation, entire staining, isolation with acid, denudating ovule, transparence, pressuring cover glass and observation. We had observed some circumstances of embryo sacs and macrospore mother cell from sugaxbeet, Aztec marigold （Tagetes erecta L.） and Common Petunia（Petunia hybrida Vilm.）. The method is simple, direct, easily handled and lower cost.
出处
《中国糖料》
2006年第2期33-34,共2页
Sugar Crops of China
基金
国家转基因植物研究与产业化项目(G99-A-014)资助。
关键词
甜菜
胚囊
压片法
Sugarbeet
Embryo sacs
Pressure cover glass method
