β-环糊精与中性红-铜(Ⅱ)配合物的相互作用

Interaction Between β-cyclodextrin and Nature Red-Cu(Ⅱ) Complexes

采用UV-Vis光谱法研究了在pH=7．00的缓冲溶液中中性红（NR）-铜（Ⅱ）配合物与β-环糊精（β-CD）的结合作用。NR形成金属配合物NR-Cu（Ⅱ）后，结合β-CD的数目大大增加，体现了金属配合物与β-CD作用的特殊性。NR-Cu（Ⅱ）金属配合物与β-CD作用迅速，作用体系稳定，并建立了以NR-Cu（Ⅱ）配合物测定β-CD的分光光度法。NR-Cu（Ⅱ）-β-CD包合缔合物的结合比为nNR：nCu（Ⅱ）：nβ-CD=1：2：35，表观摩尔吸光系数ε=1．74×10^4 L·mol^-1·cm^-1，NR-β-CD配合物与β-CD作用的表观结合常数K=1．97×10^119。β-CD的浓度在0～199．5mg·L^-1范围内遵守比耳定律（R=0．9956），桑德尔灵敏度为0．066μg·cm^-2，样品加标回收率为99．3％～100．8％，相对标准偏差为1．40％～2．26％。

The interaction of Nature Red（NR） - Cu（Ⅱ） with β - cyclodextrin（β - CD） was studied by UV - Vis spectrophotometric method in buffer solution （pH = 7.00）. The formation of complexes NR - Cu（Ⅱ） far and away increases the count of incorporate β - CD. This is the peculiarity of interaction between β - CD with NR - Cu （Ⅱ） complexes. Interaction between β - CD with NR - Cu （Ⅱ） is expeditious. Impact system is equable. Mcasurement methodology of β - CD is established. The apparent molar absorptivity of ε = 1.74×10^4 L·mol^-1·cm^-1; Conditional constants were defined, nNR ： nCu （Ⅱ） ： nβ-CD = 1 ： 2 ： 35; Condition combine constant of NR - Cu （Ⅱ） and β - CD K = 1.97×10^119; Beer＇s law was obeyed in the range of 0～199.5 mg·L^-1 and the sandell sensitivity is 0.066 μg·cm^-2 for the determination of β - CD（R = 0.9956）; The recovery of the method for the deterruination and the relative standard deviation of β - CD were 99.3%～100.8% and 1.40%～2.26%.