腹腔内注射内皮素-1对硫酸镁引起腹痛的影响

Effects of Intraperitoneal Endothelin-1 Injection on Visceral Pain Induced by Magnesium Sulfate

目的探讨腹腔内注射内皮素-1对硫酸镁引起腹痛的影响。方法对照组小鼠腹腔注射生理盐水0.2ml,实验组小鼠注射0.625μgET-1,观察其行为学并记录扭体反应情况,30min后两组均注射0.05mol/L硫酸镁0.2ml,并进行行为学观察。结果对照组腹腔注射硫酸镁后扭体次数为(4.5±3.7),实验组注射ET-1后的扭体次数为(2.7±2.6),注射硫酸镁后的扭体次数(7.2±5.4),与对照组相比差异显著(P<0.05)。实验组注射ET-1后的扭体次数与注射硫酸镁后的扭体次数没有相关关系(P>0.5)。结论ET-1可增加硫酸镁引起的扭体次数,但ET-1引起的扭体次数与随后硫酸镁引起的扭体次数并没有相关关系。

Objective To study the effects of intraperltoneal endotheUn-1 （ET-1） injection on magnesium sulfate induced visceral pain in mice. Method Normal saline （0.2 ml） was injected intrapcritoneally into control mice. Animals in the experimental group received 0.625 μg ET-1. Thirty minutes later, mice in both control and experimental groups were injected with magnesium sulfate （0.05 mol/L in 0.2 ml）. Visceral pain, as judged by the number of convulsion, was then mnnitored. Result The number of convulsion in P〈0.05. mice receiving magnesium sulfate, ET-1, and ET-1 then magnesium sulfate was （4.5±3.7）, （2.7±2.6）, and （7.2±5.4）. Versus control group （P 〈 0.05）, respectively. The number of convulsion in mice receiving ET-1 was not correlated with the number of convulsion induced by magnesium sulfate （P 〉 0.5）. Conclusion ET-1 may increase the number of magnesium sulfate-induced convulsion. There is no correlation between the number of convulsion induced by ET-1 and that subsequently induced by magnesium sulfate.