座壳孢及其相近属PCR-RFLP分析(英文)

Analyses of the Genus Aschersonia and Related Genera Using 28S rDNA RFLP Technique

利用核糖体28S rRNA基因的RFLP对18个昆虫病原真菌(座壳孢、轮枝菌和白僵菌属)菌株进行了分析。用4种限制性内切酶对PCR产物进行了消化并采用UPGMA法对扩增产物的限制性图谱构建了亲缘图。结果表明,属间、种间的多态性高于同种不同菌株间的多态性,并可以清楚地将座壳孢属不同种区分开来,而且轮枝菌不同菌株间存在着遗传差异。轮枝菌与白僵菌间的亲缘关系比其与座壳孢近。

Eighteen isolates of entomopathogenic fungi, including Aschersonia, LecaniciUium and Beauveria were analyzed by RFLP（restriction fragment length polymorphism） analysis of a 28S rRNA gene fragment. The data obtained by four different endonucleases were used to construct phenograms based on the UPGMA algorithm. Polymorphisms were found between genera and species, with fewer among isolates within species. PCR-RFLP within the 28S rRNA gene was sufficient to distinguish between Aschersonia species. RFLP data showed genetic diversity in Lecanicillium isolates at an infraspecific level. The UPGMA analysis results indicated the closer taxonomic affinity of Lecanicillium to Beauveria compared to Aschersonia.