青藤碱脂质体包封率的测定

Study on determination of entrapment efficiency of sinomenine liposomes

目的:建立青藤碱脂质体包封率的测定方法。方法:分别采用凝胶过滤法、微柱离心法、透析法分离脂质体和游离药物,并进行方法学考察,优化包封率测定条件。结果:凝胶过滤法能有效地将青藤碱脂质体与游离青藤碱分离,柱回收率测定结果为98.8%,3次包封率测定的平均值为64.9%,RSD为2.67%。结论:凝胶过滤法较为简便,结果重复性好,适于青藤碱脂质体包封率的测定。

Objective： To establish an HPLC method for the determination of entrapment efficiency of sinomenine liposomes. Method： The lipesomes and dissociated drugs were separated by sephadex filtration, mini-colunm centrifugation and dialysis. The methodology study and the optimization of determining condition were carried out at the same time. Result： Sephadex fdtration could effectively separate the sinomenine lipesomes from dissociated sinomenine. The column recovery was 98.8%, the average entrapment efficiency of three tests was 64.9%, RSD 2.67%. Conclusion： The method was simple, exact, and had a good reappearance. It can be used to examine the entrapment efficiency of sinomenine lipesomes.