当归多糖铁理化性质的初步研究

Preliminary studies on physicochemical properties of Angelica sinensis polysaccharide-iron complex

目的：对当归多糖铁的某些理化性质进行初步研究。方法：以高价铁盐的鉴别反应为依据，通过与氢氧化铁进行比较确定当归多糖铁的定性鉴别反应；置换碘量法测定当归多糖铁的铁含量；以氢氧化钠滴定当归多糖铁，根据当归多糖铁的水解情况，判断它在生理条件下的稳定性；采用邻菲罗啉比色法，对当归多糖铁进行还原性实验。结果：当归多糖铁显示高价铁盐的定性鉴别反应；铁含量介于10％-40％，水溶性与铁含量有关；水解性实验表明，当归多糖铁在pH3～12性质稳定，无沉淀出现；还原性实验表明，在37℃的温度下，6h内当归多糖铁中的Fe（Ⅲ）基本被还原剂抗坏血酸完全还原为Fe（Ⅱ）。结论：当归多糖铁可以采用高价铁的鉴别反应进行定性鉴别，铁含量在20％-25％有较好的水溶性，生理条件下理化性质稳定。

Objective： To study some physicochemical properties of Angelica sinensis polysaccharide-iron complex （APC）. Method： Based on the qualitatively identified reactions of iron （ Ⅲ ）, the qualitatively identified reactions of APC were found out by comparing with ferric hydroxide. The content of iron （Ⅲ） APe was determined with iedometry. The stability of APC under physiological pH conditiorts was judged by titrating APC with sodium hydroxide. The deoxidization of APC was tested with colorimetric analysis. Result： APC showed the qualitatively identified reactions of iron （ Ⅲ ）. The content of iron （ Ⅲ ） in APC ranged from 10% to 40%, and the water-solubility of APC was related to the content of iron （Ⅲ） The complex was stable at physiological pH from3 to 12, without precipitation and dissociation. At 37 ℃, Fe （ Ⅲ ） in the complex was completely reduced to Fe （ Ⅱ ） by ascorbic acid in about 6 hours. Conclusion： APC can be qualitatively identified by using the qualitatively identified reactions of iron （ Ⅲ ）. When its iron （ Ⅲ ） content is within 20%-25%, APC has a better ability to dissolve in water. And the complex is stable under physiological pH conditions.