制首乌对叠氮钠脑内灌流大鼠纹状体细胞外液乙酰胆碱和胆碱水平的影响

Effect of prepared Polygonum multiflorum on striatum extracellular acetylcholine and choline in rats of intracerebral perfusion with sodium azide

目的:探讨制首乌对叠氮钠脑内灌流大鼠脑保护作用的机制。方法:大鼠分成6组:对照组、模型组、制首乌组、都可喜组和制首乌+都可喜组。应用脑内微透析技术,高效液相色谱-柱后固定化酶反应器-电化学检测器动态监测清醒自由活动大鼠纹状体细胞外液乙酰胆碱(ACh)和胆碱(Ch)水平的变化。结果:在动态观察期间,对照组大鼠纹状体细胞外液ACh和Ch水平较稳定。模型组的ACh水平明显低于对照组;3个给药组的ACh水平则分别在一些时间点显著高于模型组。模型组的Ch水平则明显高于对照组;3个给药组的Ch水平在一些时间点显著低于模型组。制首乌的作用与阳性药都可喜相近。结论:制首乌通过升高ACh水平、改善Ch摄取,提高受损的胆碱能神经功能,来达到脑保护作用。为其作为脑保护剂用于治疗老年人常见的神经退行性疾病所致的痴呆提供了实验依据。

Objective： To investigate the protection mechanism of prepared Polygonum multtiflorum （PPMT） in rat brain with sodium aside （NaN3） perfusion. Method： Rats were divided into six groups： control, model, PPMT, Duxil and PPMT ＋ Duxil groups. The intracerebral microdialysis and high performance liquid chromatngraphy-post column Immobilized enzyme reactor-electrochemical detection were used to continuously measure extracellular acetylcholine （ACh） and choline （Ch） levels in striatum of freely moving awake rats. Result： The extracellular Ach, Ch levels of striatum stayed stable in the control group during the whole observing period, but the ACh levels in the model group were lower significant than that in the control group. The Ach levels of three drug groups were respectively higher significant than that of model group at some time points. While the extracellular Ch level in striatum of the model group increased singnificantly compared with the control group. The Ch levels of the three drug groups were lower significant than that of the model group respectively at certain time points. The effects of PPMT were similar with that of Duxil. Conclution： The prepared P.multiforum can improve the impaired cholinergic nerve function to exert the effects of brain protection by elevating extracellular Ach level and improving uptake of extracellular Ch. It may provide the experimental evidence to support the idea that P. multiflorum could be brain protective drug to treat retrogressive disease such as dementia.