调脉饮注射液抗心律失常的实验研究

Study on effect of Tiaomaiyin injection on experimental arrhythmia

目的:研究调脉饮注射液的抗实验性心律失常作用,并初步探讨其作用机制。方法:动物随机分为空白对照组、阳性药对照组和调脉饮注射液大、小剂量组,观察:对小鼠吸入氯仿所致室颤的保护作用;大鼠经20%乌拉坦6 mL.kg-1静脉麻醉后开胸,从颈静脉匀速滴入1.0μg.mL-1.min-1乌头碱溶液,豚鼠麻醉后从颈静脉匀速滴入10μg.mL-1.min-1哇巴因溶液,观察出现室性期前收缩、室性心动过速、室颤和心搏停止的时间,然后换算为乌头碱和哇巴因的累积量;大鼠麻醉后开胸,结扎冠状动脉5 min后恢复冠脉供血,造成缺血再灌注,观察心律失常持续时间。实验结束后取心脏制成10%匀浆,测定超氧化物歧化酶(SOD)和丙二醛(MDA)含量。结果:调脉饮注射液可以提高室颤阴性率,降低小鼠室颤的发生率(P<0.01);推迟大鼠室性早搏、室速及室颤的发生时间,提高乌头碱的累积用量,具有抗乌头碱心律失常(P<0.05)的作用;推迟豚鼠室性早搏的发生时间,提高哇巴因的累积用量,具有抗哇巴因心律失常(P<0.05)的作用;对心肌缺血再灌注所致心律失常有保护作用,能够缩短心律失常的持续时间,并能明显提高心肌SOD的活性。结论:调脉饮注射液能对抗实验性心律失常,对心肌缺血再灌注所致心律失常有保护作用,其作用机制可能与抑制脂质过氧化,减少自由基损伤有关。

Objective： To study the effect of Tiaomaiyin injection on the experimental arrhythmia for analyzing its tmderlying mechanism in the treatment of cardiovascular disease. Method： Experimental animals anesthetized with 20% urethane （6 mL·kg^- 1 ） were evenly randomized into control group, positive control group, low-dose and high-dose Tiaomaiyin group. The rate of ventricular fibrillation （VF） chloroform-induced in mice, and the epoch of ventricular extrasystole （VE）, ventricular tachycardia （VT）,VF and cardiac arrest （CA）, actonitine-induced in rats （1.0μg·mL^-1·min^-1）, and vabain-induced in guinea pigs （10μg·mL^-1·min^-1）, were detected respectively. The result loas converted into cumulative dosage of actonitine or vabain. In ischemia-reperfusion model in rats, the duration of arrhythmia and activity of superoxide dismutase（SOD） and malondialdehyde （MDA） were detected. Result： After venous injection of Tiaomaiyin, VF in mice was lower significantly （P 〈 0.01）, VE, VT, VF in rats and VF in guinea pigs were lowered considerably （P 〈 0.05）. The duration of arrhythmia in ischemia-repeffusion model was reduced considerably （P 〈 0.05）, and the activity of myocardial SOD was raised significantly （P〈 0.01）. Conclusion： Tiaomaiyin shows the reduction of experimental arrhythmia and protect effect to ischemia-reperfusion injury of the heart, which indicates that the effect mechanism may have the relationship with inhabition of lipid peroxidation and damnification of the free radical.