摘要
目的:观察钩藤总碱预处理对急性缺氧后海马神经元电压门控性Na+电流的影响。方法:原代培养大鼠海马神经元,分为常氧、急性缺氧和钩藤总碱预处理进行实验。采用膜片钳全细胞记录技术,检测海马神经元钠电流(INa)的变化。结果:急性缺氧后,细胞INa幅度明显降低,INa阈值右移;而经钩藤总碱预处理的海马神经元INa的降低幅度明显减轻。结论:钩藤总碱预处理提高了海马神经元对急性缺氧的耐受性,对神经元有显著保护作用。
Objective： To investigate the effect of Uncaria rhynchophylla total alkaloids （RTA） pretreatment on the voltage-gated sodium currents of the rat hippocampal neurons after acute hypoxia. Method： Primary cultured hippocampal neurons were divided into RTA pretreated and non-pretreated groups. Patch clamp whole-ceU recording was used to compare the voltage-gated sodium current amplitude and threshold with those before hypoxia. Result： After acute hypoxia, sodium current amplitude was significantly decreased and its threshold was upside. RTA pretreatment could inhibit the reduction of sodium current amplitude. Conclusion： RTA pretreatment alleviates the acute hypoxiainduced change of sodium currents, which may be one of the mechanisms for protective effect of RTA on cells.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第9期763-765,共3页
China Journal of Chinese Materia Medica
关键词
钩藤
缺氧
海马神经元
膜片钳
Uncaria rhynchophylla
hypoxia
hippocampal neurons
patch clamp
