多肽合成新药胸腺素α_1的凝胶电泳分析方法研究

Analysis of low molecular peptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

目的研究SDS-PAGE分析小分子合成多肽胸腺素α1的方法。方法采用Tricine-SDS-PAGE系统,降低三层胶的浓度,制成16%T(C=3%)的分离胶,10%T(C=3%)的成层胶与4%T(C=3%)的积层胶,胶中不加尿素,且不需银染。结果SDS-PAGE电泳谱图显示,合成多肽Tα1与标准品日达仙对应位置有一明显条带,成像清晰。结论该分析方法简单、快速、灵敏、重复性好,适用于小分子多肽的分离纯化鉴定。

Objective To investigate a sensitive method of electrophoresis for analysis of low molecular weight peptides, such as thymosin α1 .Methods Trieine- SDS- PAGE was used. The concentration of separating gel, ‘spacer＇ gel,and stacking gel were 16% T（C = 3 % ）, 10 % T（C = 3 % ）, and 4 % T（C = 3 % ）, respectively. Results The SDS - PAGE showed that the low molecular peptides Tα1 formed an obvious band even without silver staining. Conclusion The established method is simple, rapid, sensitive, reproducible, and applicable to analysis of low molecular peptides.