摘要
自从1975年Caul等报告在患有胃肠炎的病人粪便中发现了冠状病毒样颗粒以来,包括Caul本人在内的许多研究者都试图用人工方法分离培养人肠道冠状病毒。Caul曾先后用原代人胚肾细胞和人胚肠道器官培养人肠道冠状病毒,并用免疫荧光和电镜等方法予以证明。1981年lapcrte等报道,HRT-18细胞可用于培养人和狗的肠道冠状病毒。但不能长期传代,而且未获超薄切片等有力证据。Patel、Mortensen等曾用包括HRT-18细胞在内的传代和继代细胞进行培养,均未获成功。本实验室在对多种细胞筛选后发现,人胚肺二倍体细胞对人肠道冠状病毒比较敏感。现将我们用这株细胞对人肠道冠状病毒分离鉴定的结果报告如下。
Stool-supernatant from 8 diarrhoea cases were cultured in 2BS cell line treated with trypsin for the isolation of human enteric corona virus. Coronaviruses were successfully isolated from 4 of them. The isolate 85-2 was identified by electron microscopic negative staining method, ultra-thin section, test of stability to acid and ether, initialization and cross- nutralization tests, and type of nucleic acid. These results indicate that the virus isolated in cell culture is a human enteric corona virus.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第1期77-80,共4页
Chinese Journal of Virology
关键词
肠道冠状病毒
病毒分离
Human enteric coronavirus Virus isolation
