单克隆抗体对肾综合征出血热病毒感染乳鼠的保护作用

PROTECTION OF MONOCLONAL ANTIBODY FOR HFRS VIRUS INFECTED MICE

用肾综合征出血热病毒(Hemorrhagic fever with renal syndrom virus,HF-RSV)陈株感染乳鼠,检测了25株单克隆抗体(McAb)对感染乳鼠的保护作用.结果表明,其中3株McAb被动使用后,对感染乳鼠有明显的保护作用,保护力大小与病毒感染剂量、抗体使用剂量及使用时间均有关.分析了McAb在活体内的保护作用与其体外血凝抑制活性及中和活性的关系.

A method that inoculated lethal dose of hemorrhagic fever with renal syndrome virus ( HFRSV ) into suckling mice intraperitoneally for measuring the protective effect of specific antibody to infected mice was studied. Analysing the signs of mice after inoculation and detection of H FRSV antigen, results showed that this method was feasible with merits of object, stability and easy to observation. Results also showed that passive administration of specific antibfdy acted strong on preventing mice from HFRSV infection and its effectiun was related with the dose of HFRSV inoculation,dose of antibody and time of antibody administration. This suggested that antibody may be a good reagent used for control clinical HFRS and was better by using earlier and enough. By measurement of anti-HFRSV McAbs,3 of 25 showed gocd protective effect. The interrelation of activity on HIT, NT and protection of McAbs was also analysed in this study.