ABC-ELISA检测鸡新城疫病毒

DETECTION OF NEWCASTLE DISEASE VIRUS ( NDV ) BY ABC-ELISA

建立了检测鸡新城疫病毒的ABC-ELISA方法。应用此法测定了Lasota株、Mukt-eswar株和细胞培养的新城疫强毒的效价,结果较满意。比较试验证明,ABC-ELISA的敏感性比常规ELISA高2～16倍,比HA法高4～64倍。检测感染鸡口腔和泄殖腔棉拭材料,检出率分别为83.3％和100％,比常规ELISA分别提高33.3％和16.7％,而HA则检不出。该法特异性好,能被特异性抗体和亲和素所阻断,对传染性支气管炎病毒、鸡痘病毒、鸭瘟病毒均不发生交叉反应。重复性亦好,同一样本先后经多次测定,结果基本一致。

A new ABC-ELISA method was developed to assess the titer of NDV Lasota strain, Mukteswar strain(I)and virurent NDV in cell culture. The sensitivity of ABC-ELISA was 2-16 times and 4-64 times higher than that of conventional ELISA and HA tests respectively. In testing NDV in oral and cloacal swabs, positive detection rates of ABC-ELISA were 83.3% and 100% respectively, being 16.7% and 33.3% higher than that of conventional ELISA. Positive detection was not obtained by HA test.The specificity of ABC-ELISA was excellent. Its reactivity could be stopped by specific antibody or avidin, and no cross reaction with infectious bronchitis virus, avianpox virus, duck plague virus was noted. This method proved to be reproducible, its results were almost identical when the same sample was assayed several times in different periods.