南京地区扁豆病毒病主要毒原的鉴定

INDENTIFICATION OF STAPLE VIRUSES OF DOLICHOS IN NANJING

从南京农业大学校园内的感病扁豆上,得到DL-4和DL-6两个病毒分离物。 DL-4的特性是:感病扁豆表现为系统环斑。接种的6科21种植物中,有4科12种可被侵染。不被桃蚜、豆蚜以非持久方式传播。体外失毒温度为50～60℃,稀释限点为10^(-2)～10^(-3),保毒期2天。病毒粒体为球形,直径28～30nm。提纯的DL-4紫外吸收值D_(260/280)为1.66。3种免疫学测试表明,与黄瓜花叶病毒Y株系抗血清呈阳性反应。故鉴定为黄瓜花叶病毒(CMV)。 DL-6的特性是:感病的扁豆表现为系统花叶或斑驳、摩擦接种可系统侵染大豆、蚕豆,在苋色藜上产生褪绿斑,在菜豆上产生枯斑。可由桃蚜、豆蚜以非持久方式传播。体外失毒温度为60～65℃,稀释限点为10^(-2)～10^(-3),保毒期2～4天。病毒粒体平均长度764nm。扁豆病叶组织超薄切片中可见风轮状及束状内含体。三种免疫学测试指标皆表明,与大豆花叶病毒呈阳性反应。故鉴定为大豆花叶病毒(SMV)。

Isolates DL-4 and DL-R were obtained from dolichos ( Dolichos lablab L. ) in Nanjing Agricultural University.DL-4 properties: 12 species of 4 familes out of 25 species of 6 familes plants could be infected by sap inoculation. Myzus persicae and Aphis cra-ccivora failed to transmit DL-4 in non-persistent manner. Its physical properties in crude sap were, thermal inactivation point 50-60℃, dilution end point 10-2-10-3,and longevity in vitro 2 days. The viral particles were isometric, 28-30nm in diameter. The UV absorbance of OD 260/280 was l. 66. DL-4 gave positive reaction with CMV-Y antiserum in 3 kinds of immuno-logical tests. on the basis of these characteristics, DL-4 was identified as cucumber mosaic virus ( CMV ) .DL-6 properties, Soybean, broad bean could be systemically infected by sap inoculation. DL-6 could cause chlorotic lesions on inoculated leaves of Chetopodium amaranticolor and lesions on bean ( Top crop ) . DL-G was easily transmitted by Myzus persicae. Aphis craccivora in a nonpersistent manner. Its physical properties in crude sap were, thermal in activation point 60-65℃,dilution end point 10-2 -10-3,and longevity in vitro 2-4 days. The average length of flexuous particles was 764nm. Pinwheel and boundle in-dusions were found in the cytoplasm of leaf tissues of infected dolichos. DL -6 could produce reaction with SMV antiserum in 3 kinds of immunological tests. According to these characteristics, DL-6 was identified as soybean mosaic virus ( SMV ) .