钻天柳腋芽组培快繁技术的研究

Rapid-propagation of Chosenia arbutifolia by axillary buds

钻天柳是杨柳科钻天柳属唯一的一个种，是亚洲东北部的珍稀树种，已被列为国家重点保护植物。钻天柳的种子非常小、非常轻，天然下种更新非常困难，而且扦插繁殖的生根能力很差，因此，研究钻天柳优树的组织培养技术是非常必要的。本文详细介绍了钻天柳幼枝的腋芽再生完整植株的组织培养技术：基本培养基为MS培养基，初始培养基附加BA0.20＋NAA0．20，芽形成培养基附加BA0．50＋NAA0．20，枝形成增殖培养基为1／2MS＋BA0．30＋NAA0.05，生根培养基为1／2MS＋IBA0．50＋pvp20．00。该优化的培养系统繁殖系数达10-30，生根率达90％以上。

Chosenia arbutifolia is the only species of Chosenia genus, Salicaceae. It is a rare and useful tree in the northeast of Asia. Now this tree species has been listed China National Priority Protection Plant. Its seeds are very smaller and lighter, natural regeneration by seed shedding is very difficuhy. In addition, the root - forming ability in the vegetative propagation is inferior. Therefore, it is worthwhile to develop a tissue culture technique for the rapid propagation of selected plus trees. This paper introduces the culture procedure to the plant.lets which were successfully regeneration from axillary buds of Chosenia arbutifolia excised from young seedlings. Murashige and Skong＇s medium was used as the basic culture medium. Growth was initiated on MS medium containing 0.2mg/LBA and 0.2mg/LNAA. Bud formation was induced using MS medium supplemented with 0.5mg/L BA and 0.2mg/L NAA. Development of shoots was achieved on a 1/2 MS medium containing 0.3mg/L BA and 0.05mg/L NAA. The medium used for inducing root formation was the same as the above, but with 0.5mg/L IBA and 20 mg/L PVP. The multiplication ecefficient of the optimization medium system is 10 - 30, and root - forming rate is above 90%,