1988年上海甲型肝炎暴发流行中从毛蚶分离到甲型肝炎病毒

ISOLATION OF HEPATITIS A VIRUS FROM CLAMS DURING A SEVERE OUTBREAK OF HEPATITIS A IN SHANGHAI, 1988

1988年初,上海市发生甲型肝炎暴发流行,流行病学调查证明,这次流行系市民食用未经煮熟的毛蚶所致。本文应用电镜、细胞培养技术,从上海市售毛蚶产地——江苏省启东县采集的毛蚶标本中直接检测和分离到3株甲型肝炎病毒(HAV)。该3株病毒在人二倍体细胞上可稳定传代,感染满度达7 0～9 0TCID50/ml。故从病原学角度证实,污染毛蚶可携带大量甲型肝炎病毒,并且酿致暴发流行。

Epideiniological investigation proved the cause of viral hepatitis A outbreak in Shanghai was due to the eating of raw clams ( Area subcrensta Lisckke ) which were collected from Qidong, Jiangsu Province, 1988. The results of etiological study showed that both 'full' and 'empty' hepatitis A virus particle clusters were found by immune electron microscope in clam extracts collected from Qidong, in addition,Rota virus-like and coro-navirus-like particles, as well as different forms of bacteria could also be observed. Three strains of hepatitis A virus were isolated in Frhk4 and human diploid lung fibroblast ( HDLF ) 2BS and KMB17 cell cultures, and two of them were identified by immunofluorescent blocking test, neutrali-zation test and immuno-electron microscope examination. Both strains were further proved to be hepatitis A virus.The newly isolated hepatitis A virus has been stably passed in HDLF cells with titers of 7.0 to 9.0 TCID50/ml. These resuhs demonstrated that clams were contaminated by hepatitis A virus from their living enviro-me nt.