脑卒中急性期血清白介素-10和外周血T淋巴细胞Ag-NORs的动态变化

Changes of serum IL-10 and Ag-NORs of T lymphocytes in patients with acute cerebral stroke

目的研究脑卒中急性期血清IL-10的动态变化规律以及细胞免疫功能变化。方法选择3d内首次发病患者,IL-10采用ELISA方法于发病72h内、第6～8天及第14～15天抽血检测,Ag-NORs于发病72h内、第14～15天抽血,对外周血T淋巴细胞染色分析,以核仁银染面积与细胞核面积比值(I.S%)作为Ag-NORs检测指标,对照组抽血检测1次。结果脑卒中组血清IL-10水平各时间点均较对照组明显增高(P<0.05),不同时间无明显变化(P>0.05),不同病情亦无明显差异(P>0.05)。脑卒中患者急性期Ag-NORs水平(I.S%)明显降低(P<0.01),发病后第14～15d较72h内高(P<0.01),但仍低于对照组(P<0.01)。脑梗死组同一时期重度患者Ag-NORs水平较轻度患者低(P<0.05)。结论IL-10在脑卒中急性期明显增高,参与了卒中炎性损伤的病理过程,有望成为治疗脑卒中的潜在药物。且脑卒中急性期细胞免疫功能低下。

Objective To study the changes of srrum IL-10 and Ag-NORs of T lymphocytes in patients with acute cerebral stroke. Methods Patients with first-ever acute stroke within the first 3 days after stroke onset were included. The level of serum IL-10 was measured using enzyme linked immunosorbent assay（ELISA）. The ratio of nuclear area by silver staining to nuclear area（I. S%） was as the index of Ag-NORs. Results 1）Compared with those of the control, the level of serum IL-10 was increased in the infarction group and cerebral hemorrhage group （P〈0.05） at all stages. The level of serum IL-10 was not obviously different in patients with different stages （P 〉 0.05）. 2） Compared with those of control, the level of Ag-NORs of T lymphoeytes in patients with acute cerebral stroke was decreased （P 〈 0. 01） ; the level of Ag-NORs in the second week was higher than that in 72 hours （P〈0.01）, but lower than that in control group （P 〈0.01）. The level of Ag-NORs of T lymphoeytes in patients with serious symptoms was lower （P〈 0. 05）. Conclusions 1） The level of anti-inflammatory eytokine IL-10 was greatly increased in patients with acute cerebral stroke, it might be involved in the inflammatory process of stroke, IL- 10 may become a potential therapeutic drug. 2） The cell immunological function in patients with acute cerebral stroke decreased, and may be assoeiated with the pathogenetic process of isehemic stroke.