摘要
1987年夏,从云南省西双版纳傣族自治州医院采集的无名热和脑炎病人血清和脑脊液中,曾分离到多株新环状病毒。本文报道同年从版纳州医院附近的个体屠宰场采集到猪血清13份、牛血清53份,从中共分离到20株病毒。血清学鉴定,该病毒与人血清分离的新环状病毒BANNA582株病毒的两株单克隆抗体有很强的交叉反应。电镜观察病毒为球形无囊膜颗粒,毒粒大小和结构与BANNA新环状病毒相同。20株病毒的基因组皆由12条RNA片段组成,其带形与BANNA新环状病毒完全相同。本文还就猪牛等脊椎动物传播和扩散该类环状病毒的意义进行了讨论。
In the summer of 1987, 16 virus isolates were recovered from 53 sera of catties and 4 isolates from 13 sera of pigs collected in the Dai Nationality Autonomous Prefecture of Xi Shuang Ban Na of Yunnan. All the 20 virus isolates showed strong cross reaction with two strains of monoclonal antibody against the new orbivirus, BANNA582, isolated from serum of a patient with unknown fever, by the method of ELISA. Electron microscopic examination of the virus revealed the same viral size and structure as those of human BANNA new orbiviruses. All 20 isolates possessed 12 segmented RNA genome and shared exactly the same RNA profile with that of BANNA new orbivirus isolated from human patients with unknown fever or encephalitis in Yunnan. It was suggested that the viruses obtained from catties and pigs were the same agent as the human BANNA orbivirus. The role of cattle and pigs as the vertebrate hosts of the new orbivirus in the transmission and amplification of the viruses is discussed.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第4期327-331,共5页
Chinese Journal of Virology
基金
国家自然科学基金
关键词
新环状病毒
动物宿主
BANNA new orbivirus Animal host