摘要
目的探讨RhoA在凝血酶或脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVEC)单层通透性改变中的作用。方法用免疫荧光和改良的Boydon小室分别检测HUVECs F-肌动蛋白和单层通透性;用Western blot和免疫组化分析HUVECs内RhoA的表达;用RT-PCR检测RhoA mRNA表达。结果HUVECs经凝血酶或LPS处理后,F-肌动蛋白解聚,细胞周边形成应力纤维,HUVECs单层通透性增高;RhoA蛋白和mRNA表达上调;Rho激酶抑制剂Y-27632能明显缓解上述变化。结论RhoA参与凝血酶或LPS诱导的HUVECs的F-肌动蛋白的重构和单层通透性的改变。
Objective To investigate the change of permeability of HUVECs monolayer induced by thrombin or Lipopolysaccharide （LPS） for exploring the role of RhoA. Methods The effect of thrombin or LPS on the permeability of HU-VECs monolayer was examined with the polycarbonate transwell filters and the effect of thrombin or LPS on HUVECs F-actin was observed with immunocytochemistry. The interferring action of Y-27632 （Rho kinase inhibitor） on the permeability and F-actin change induced by thrombin or LPS was also observed. The expression of RhoA protein and mRNA in HUVECs were detected by Western blotting and semi-quantitative RT-PCR. Results Thrombin or LPS induced F-actin depolymerized and significant increase in the permeability of HUVECs. Permeability and F-actin do not change significantly when Y-27632 was added. Thrombin or LPS could upregulate the expression of RhoA protein and mRNA in HU-VECs. Conclusion HUVECs monolayer permeability injury induced by thrombin or LPS is related to depolymerization of F-actin and RhoA signal pathway is involved.
出处
《基础医学与临床》
CSCD
北大核心
2006年第4期386-391,共6页
Basic and Clinical Medicine
基金
国家重点基础性研究项目(G2000057004)
