脂联素基因Gly15Gly多态性与非酒精性脂肪肝病的关系

Association of T45G Polymorphism in Exon 2 of Adiponectin Gene with Nonalcoholic Fatty Liver Disease

目的:研究脂联素基因外显子2的Gly15Gly多态性与非酒精性脂肪肝病(NAFLD)的关系。方法: 采用聚合酶链反应限制性片段长度多态法检测并比较107名NAFLD和112名对照者脂联素基因外显子2的 Gly15Gly基因型和等位基因频率,并根据体重指数(BMI)分为非肥胖组(BMI<25 kg／m2)和肥胖组(BMI≥25 kg／m2)进行分层分析。结果:非肥胖NAFLD39例和对照者72例中TT、TG、GG基因型分别为35．9％、48．6％和 48．7％、44．4％和15．4％、7％,两组等位基因频率(x2=2．09,P>0．05)和GG基因型频率(x2=0．64,P>0．05) 差异无显著性;肥胖NAFLD68例和对照者40例中TT、TG、GG基因型分别为55．9％、37．5％和30．9％、55％和 13．2％、7．5％,NAFLD组GG基因型频率高于对照组(x2=6．159,P<0．05),两组等位基因频率差异无显著性 (x2=0．941,P>0．05)。结论:脂联素基因外显子2的Gly15Gly多态性与NAFLD相关。

Objective：To study the relationship between T45G polymorphism in exon 2 of the adiponectin gene and NAFLD. Methods： T45G polymorphism in exon 2 of the adiponectin genotype and allele was analyzed using polymerase chain reaction and restriction fragment length polymorphism in 107 NAFLD patients and 112 controls divided into obese and non-obese groups according to body mass index. Results： In non-obese group, the frequencies of Tr, TG and GG genotypes were 35.9%, 48.6% and 48.7% in NAFLD vs 44.4% , 15.4%, and 7% in controls, with no significant difference in GG （x^2 = 2. 09, P 〉 0.05 ） and allele （x^2 = 0.64, P 〉 0.05 ） frequencies between the two sub-groups, while in obese group, these were 55.9% , 37.5% and 30.9% in NAFLD vs 55% , 13.2% and 7.5% in controls, with higher GG genotype frequency among NAFLD patients （x^2 = 6. 159, P 〈 0. 05 ） and no significant difference in allelic frequency between NAFLD and controls （x^2 = 0. 941, P 〉 0.05 ）. Conclusion： T45G polymorphism in exon 2 of adiponectin gene is associated with NAFLD.