豆天蛾核型多角体病毒gp41同源基因的克隆和序列分析

Cloning and Sequence Analysis of gp41 Gene of Clanis bilineata Nuclear Polyhedrosis Virus

目的对豆天蛾核型多角体病毒(CbNPV)的gp41同源基因进行克隆和序列分析,为进一步研究该病毒基因组的结构打下基础。方法从豆天蛾幼虫尸体中分离纯化了豆天蛾核型多角体病毒,提取其基因组DNA,用shotgun法构建了DNA片段的文库,并进行了全基因组测序。对CbNPV的gp41同源基因进行序列分析。结果CbNPV的gp41同源基因长度为933bp,编码310个氨基酸。氨基酸序列同源性分析结果表明,CbNPVGP41与I类NPV及II类NPVGP41的同源性分别为53%～61%和56%～73%。结论初步推测,CbNPV与II类NPV的亲缘关系可能更近。

[Objective] Aim of this research is to clone and analyses the gp41 gene of Clanis bilineata Nuclear Polyhedrosis Virus （CbNPV）, which will be helpful to obtain the molecular information of this virus. [Method] CbNPV was purified from Clanis bilineata larva, the genornic DNA was extracted, and a DNA fragment library of the virus was constructed by shotgun. The positive clones were sequenced and we analyzed the open reading frame which has high identity with the gp41 gene of most baculovirus. [Result] The gp41 gene of CbNPV is 933 bp long and encodes a protein of 310 amino acids. The amino acid sequence analysis indicated that the CbNPV GP41 has 53%-61% and 56%-73% identities with Group Ⅰ and Ⅱ NPVs GP41 proteins, respectively. [Conclusion] The result indicates that the CbNPV isolated by the authors is a novel baculovirus and the CbNPV has more closer relationship with Group Ⅱ NPVs.