单克隆抗体治疗日本脑炎病毒感染恒河猴的实验研究

Experimental Studies on the Therapy of Japanese Encephalitis Virus Infected Rhesus Monkeys by Monoclonal Antibodies

感染日本脑炎病毒(JEV)的恒河猴,出现脑炎的一系列症状与体征,终因呼吸及循环衰竭而死亡。JEV感染猴体温升到超过39℃以上后的第2天,注射单克隆抗体(McAb)制剂进行实验性治疗。结果显示,McAb经肌肉、静脉、硬脑膜下加肌肉等不同途径注射后,均有良好的疗效,对恒河猴的保护率达100％,其中以硬膜下加肌肉途径注射McAb的效果最佳。多次注射McAb,未发现任何不良反应与毒副作用。本研究表明,将鼠源性McAb用于灵长类安全可靠,若过渡到临床使用,是可行的。

After being infected with Japanese encephalitis virus (JEV), the rhesus monkeys appeareda series of symptoms and signs, and finally died of exhaustion of respiration and circulation. Two days after the temperature rised upto 39℃, the. monoclonal antibodies (McAb) were injected for therapy. The results were finer with the survival rate of 100％ in different groups treated by intramuscular, intravenous, and subdural plus intramuscular injections. Among them, subdural plus intramuscular injections of the McAb was the best one. After multiple injections of the McAb, there appeared neither anaphylaxis nor delayed allergy, without obvious changes of serum IgE levels, and did not observed any damages of the main organs. The studies proved that the application of McAb from mouse safe, and it is possible and reliable for clinical usage.