摘要
Provvidenti,R.(1980)和Green,S.K.(1985)先后用同一套鉴别寄主谱,研究了美国和我国台湾省的TuMV株系分化情况。本文用Green的方法,对由十省(市)7,982份病样中筛选出的19个TuMV主流分离物,在同一条件下进行了鉴定。结果表明,属于C_1株系的有黑_3分离物;属于C_4株系的有京_2、京_3、冀_2、宁_1(南京)、粤_1和川_1共6个分离物;属于C_5株系的有黑_1、辽_1、京_1、冀_1、沪_1、鲁_1、鲁_2共7个分离物。未检出C_2和C_3株系。黑_2、沪_2、宁_2和秦_1(陕西)共4个性状相近的分离物和冀_3分离物尚不能按Green的标准归类,暂分别定为C_(3-2)和C_6株系。此外,对Green氏方法应用于我国十字花科蔬菜TuMV株系分化研究中存在的问题作了讨论。
Turnip mosaic virus strains differentiation in the United States and Taiwan province of China were identified by Provvidenti, R. (1980)and Green, S K. (1985) with a set of the same differential hosts. Green's identification methods were applied in this experiment. Nineteen major isolates of TuMV were identified under the same controlled conditions. These nineteon isolates were identified and screened from 7982 TuMV samples collected from ten regions of China. The identifying result indicated that TuMV Hei-3 isolate belonged to C_1 strain;TuMV Beijing-2 and 3, Hebei-2, Nangjiang-1, Cuangzhou-1 and Sichuan-1 were classified as C_4 strain; seven TuMV isolates of Hei-1, Liao-1, Beijing-1, Hebei-1, Shanghai-1, Shandong-1 and 2 were all belonging to C_5 strain. There were no C_2 and C_3 strains in this test. However, four TuMV isolates of Hei-2, Shanghai-2, Nangjiang-2 and Shanxi-1 the properties of which were similar and another isolate of Hebei-3 cannot be classified according to Green's standard. They were temporarily named C_(3-2) and C_6 strains respectively. In addition, the problem to study TuMV strain differentiation on cruciferous vegetables of China with Green's methods was discussed in this paper.
关键词
鉴别寄主谱
TUMV
株系分化
株系
Differential hosts TuMV isolate Strain differentiation Strain
