摘要
本文介绍应用Vero-E_(?)细胞直接从EHF抗原阳性的褐家鼠肺中分离出EHFV-R_(36)株。形态学鉴定符合EHFV,并见到典型的颗粒性EHFV包涵体和大小形态类似的EHFV样颗粒。分离毒株经EHFV单克隆抗体分析,R_(36)株EHFV的抗原谱不同于我国的R_(22)株,它既带有家鼠型R_(22)株相似的抗原决定簇,同时又具有野鼠型抗原决定簇。
In this paper, it is reported that the result of isolated EHF virus R36 strain directly from the antigen positive lung by using Vero-E6 cell. Morphological identification is corresponding to EHF virus by EM and it is found that typical particle EHF virus inclusion bodies and size similar EHFV-like particle. By EHFV monoclonal antibody assay this virus strain was different from that of R22 in China. It has rattus R22-like antigenic determinant and apodemus 5H5 antigenic determinant.
