单纯疱疹病毒基因组的限制性核酸内切酶分析

Analysis of Herpes Simplex Virus Genomes with Restriction Endonucleases

为了获得单纯疱疹病毒(HSV)基因组的限制性核酸内切酶(RE)分析资料和选择合适的RE去研究HSV感染,用11种常用的RE对HSV两个型别的实验室标准毒株的基因组分别作了分析。比较研究的结果表明,BamHI、HpaI和PstI等RE较适于HSV的研究和分型。本研究所采用的小量提取HSV DNA的方法具有快速、简便和实用的特点,值得在HSV感染的诊断、分型和HSV分子流行病学诸研究中推广使用。

In order to obtain the materials of restriction endonuclease analysis of Herpes Simplex Virus(HSV) genomes and select suitable restriction endonuclease(RE) for studying and typing of HSV infection, the genomes of two standard strains of HSV, HSV-1 F strain and HSV-2 G strain have been digested with 11 REs. The results showed that the REs, especially BamHI, HpaI and PstI,were found to be useful for typing and studying of HSV DNA in the clinical virology and molecular virology. The quick preparation of HSV DNA(for small scale) is a simple, rapid and practical procedure, which can be used in the diagnostic virology and molecular epidemiology.